Meyaard L, Otto S A, Keet I P, van Lier R A, Miedema F
Department of Clinical Viro-Immunology, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Blood. 1994 Dec 15;84(12):4262-8.
In addition to the loss of CD4+ T cells in later stages of human immunodeficiency virus (HIV) infection, functional defects of Th cells can already be observed in early infection. Decreased interleukin (IL)-2 and interferon (IFN)-gamma production by CD4+ T cells and diminished delayed type hypersensitivity reactions are indicative for impaired Th1 responses. We studied the cytokine secretion patterns of T-cell clones (TCC) generated by mitogenic stimulation of CD4+ memory T cells. Compared with TCC from HIV-negative controls, TCC isolated from HIV-infected individuals consistently showed increased IL-4 production, often paralleled by increased IL-5 and decreased IFN-gamma production. This resulted in a decreased percentage of Th1 clones with an increase in Th0 clones. To rule out the influence of interindividual differences, we studied two individuals from whom cells were available before and after infection with HIV. Indeed, an increase in Th2 cytokine secretion was observed after HIV-infection. Loss of Th1 and enhanced Th2 responses might further curtail cellular responses resulting in deficiency of cellular immunity in HIV infection.
除了在人类免疫缺陷病毒（HIV）感染后期CD4 + T细胞数量减少外，在感染早期就已观察到Th细胞的功能缺陷。CD4 + T细胞分泌白细胞介素（IL）-2和干扰素（IFN）-γ减少以及迟发型超敏反应减弱表明Th1反应受损。我们研究了通过有丝分裂刺激CD4 + 记忆T细胞产生的T细胞克隆（TCC）的细胞因子分泌模式。与来自HIV阴性对照的TCC相比，从HIV感染个体分离的TCC始终显示IL-4产生增加，通常伴随着IL-5增加和IFN-γ产生减少。这导致Th1克隆百分比降低，Th0克隆增加。为了排除个体差异的影响，我们研究了两名在感染HIV之前和之后都有可用细胞的个体。确实，在HIV感染后观察到Th2细胞因子分泌增加。Th1的丧失和Th2反应的增强可能会进一步削弱细胞反应，导致HIV感染中细胞免疫缺陷。
