Guimbaud R, Abitbol V, Bertrand V, Quartier G, Chauvelot-Moachon L, Giroud J, Couturier D, Chaussade D C
Service d'Hépatogastroentérologie, Hôpital Cochin, 27, rue du Faubourg-Saint-Jacques, 75014 Paris, France.
Eur Cytokine Netw. 1998 Dec;9(4):607-12.
The pleiotropic cytokine leukemia inhibitory factor (LIF) possesses proinflammatory properties in common with tumor necrosis factor (TNF-alpha), interleukine (IL) -1 and -6, such as the induction of acute phase protein synthesis. LIF may have chemotactic activity through the induction of IL-8 production. LIF is produced by normal and tumoral cells and appears to facilitate in vivo rat colon carcinoma cells growth. Inflammatory bowel diseases, ulcerative colitis (UC) in particular, are histologically characterized by the infiltration of the colonic mucosa with activated neutrophils, macrophages and lymphocytes. Cytokines with their inflammatory as well as their regulatory activities may play a role in the perpetuation and possibly the initiation of inflammation in this disease and its local and/or systemic complications. Moreover, colorectal cancer is a late well identified complication in patients with long standing inflammatory bowel disease, UC in particular. Taken together, these results suggest that LIF could be involved in tumorigenic and/or metastatic processes of colorectal cells in UC patients. The aims of the present study was to quantify and to compare the colonic and systemic productions of LIF in UC patients. We showed for the first time in patients with UC, a high local production of LIF well correlated with IL-8 production. We also analyzed the effect of LIF on a human colon carcinoma cell line HT29. We demonstrated that LIF stimulated HT29 cell growth in a dose dependent-manner. These results suggest that LIF may play a critical role in the susceptibility of colonic host cells to tumor growth in patients with UC.
多效细胞因子白血病抑制因子（LIF）具有与肿瘤坏死因子（TNF-α）、白细胞介素（IL）-1和-6相同的促炎特性，如诱导急性期蛋白合成。LIF可能通过诱导IL-8产生而具有趋化活性。LIF由正常细胞和肿瘤细胞产生，似乎促进体内大鼠结肠癌细胞生长。炎症性肠病，尤其是溃疡性结肠炎（UC），在组织学上的特征是结肠黏膜有活化的中性粒细胞、巨噬细胞和淋巴细胞浸润。具有炎症及调节活性的细胞因子可能在该疾病炎症的持续存在以及可能的起始过程及其局部和/或全身并发症中起作用。此外，结直肠癌是长期炎症性肠病患者，尤其是UC患者中一种已明确的晚期并发症。综上所述，这些结果表明LIF可能参与UC患者结肠细胞的致瘤和/或转移过程。本研究的目的是量化和比较UC患者结肠和全身LIF的产生情况。我们首次在UC患者中发现，LIF的局部产生量很高，且与IL-8的产生密切相关。我们还分析了LIF对人结肠癌细胞系HT29的影响。我们证明LIF以剂量依赖的方式刺激HT29细胞生长。这些结果表明LIF可能在UC患者结肠宿主细胞对肿瘤生长的易感性中起关键作用。
