关于真核生物中的小基因组：两种脊椎动物寄生虫微孢子虫（原生动物）的分子核型。
On small genomes in eukaryotic organisms: molecular karyotypes of two microsporidian species (Protozoa) parasites of vertebrates.
作者信息
Biderre C, Pages M, Metenier G, David D, Bata J, Prensier G, Vivares C P
机构信息
Protistologie moléculaire et cellulaire des parasites opportunistes, URA CNRS 138, UBP, Biologie A, Aubière, France.
出版信息
C R Acad Sci III. 1994 May;317(5):399-404.
Pulsed field gel electrophoresis (PFGE) was used to separate chromosome-sized DNA from two species of microsporidia of fishes. The molecular karyotype of Glugea atherinae exhibits 16 DNA bands from 420 to 2,700 kb, and that of Spraguea lophii 12 bands from 230 to 980 kb. Until now they represent respectively the largest and the smallest genomes visualized for microsporidia: 19.5 Mb for G. atherinae and 6.2 Mb for S. lophii (the smallest nuclear genome in eukaryotic organism). We have analysed separately five strains of G. atherinae (individual cysts), with this technique. The electrophoretic spectra are the same for these strains, except for the absence of the 2,380-kb band in one case. Therefore, the karyotype seems to be rather well conserved for this species.
脉冲场凝胶电泳（PFGE）被用于分离两种鱼类微孢子虫的染色体大小的DNA。艾氏格留虫的分子核型显示出16条DNA条带，大小在420至2700 kb之间，而洛氏斯普拉克虫的分子核型有12条带，大小在230至980 kb之间。到目前为止，它们分别代表了微孢子虫中可视化的最大和最小基因组：艾氏格留虫为19.5 Mb，洛氏斯普拉克虫为6.2 Mb（真核生物中最小的核基因组）。我们用这项技术分别分析了五株艾氏格留虫（单个包囊）。除了在一个样本中没有2380 kb的条带外，这些菌株的电泳图谱是相同的。因此，该物种的核型似乎相当保守。
Zotero 插件