Seltmann G, Voigt W, Beer W
Robert-Koch-Institut des Bundesgesundheitsamtes, Bereich Wernigerode, Germany.
Epidemiol Infect. 1994 Dec;113(3):411-24. doi: 10.1017/s0950268800068424.
Eighty-nine Salmonella enteritidis phage type 25/17 strains isolated from a localized outbreak in the German state Nordrhein-Westfalen (outbreak NWI) could not be further differentiated by biochemotyping and plasmid pattern analysis. They were submitted to a complex typing system consisting of modern physico-chemical analytical procedures. In lipopolysaccharide pattern analysis the strains proved to be homogeneous. In multilocus enzyme electrophoresis, outer membrane and whole cell protein pattern (WCPP) analysis, and Fourier-transform infrared (FT-IR) spectroscopy (increasing extent of differentiation in the given order) strains deviating from each basal pattern were found. The extent of correspondence in these deviations was satisfactory. Forty-six strains of the same sero- and phage type, however, obtained from different outbreaks, were additionally typed. The results obtained with them indicate that the data of the first group were not restricted to strains from outbreak NWI, but of general validity. It was found that both WCPP and FT-IR represent valuable methods for the sub-grouping of bacteria.
从德国北莱茵-威斯特法伦州局部疫情（疫情NWI）中分离出的89株肠炎沙门氏菌25/17型菌株，无法通过生化分型和质粒图谱分析进一步区分。它们被提交到一个由现代物理化学分析程序组成的复杂分型系统中。在脂多糖图谱分析中，这些菌株被证明是同质的。在多位点酶电泳、外膜和全细胞蛋白图谱（WCPP）分析以及傅里叶变换红外（FT-IR）光谱分析（按给定顺序区分程度增加）中，发现了偏离每种基础图谱的菌株。这些偏差中的对应程度令人满意。然而，另外对从不同疫情中获得的46株相同血清型和噬菌体类型的菌株进行了分型。用它们获得的结果表明，第一组数据并不局限于来自疫情NWI的菌株，而是具有普遍有效性。结果发现，WCPP和FT-IR都是细菌亚分组的有价值方法。
