Wang N L, Kiyohara H, Matsumoto T, Otsuka H, Hirano M, Yamada H
Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
Planta Med. 1994 Oct;60(5):425-9. doi: 10.1055/s-2006-959524.
Anti-sera against a complement-activating pectin (AR-2IIb), which was purified from the roots of Angelica acutiloba Kitagawa, were obtained by immunization of rabbits, and a polyclonal anti-AR-2IIb antibody of the IgG class was purified by affinity chromatography on AR-2IIb-immobilized Sepharose and Protein G-Sepharose. Periodate oxidation of AR-2IIb significantly reduced its inhibitory activity on the reactivity of AR-2IIb to anti-AR-2IIb-IgG, but pronase digestion of AR-2IIb did not affect its inhibitory activity. Other pharmacologically active pectins from A. autiloba, Bupleurum falcatum, and Glycyrrhiza uralensis and the complement-activating pectic arabinogalactan from A. autiloba also showed significant inhibitory activities on the reactivity of AR-2IIb to anti-AR-2IIb-IgG, but these inhibitory activities were lower than that of AR-2IIb. Other pectins, polygalacturonic acid, arabinogalactan, galactan, and araban tested had negligible inhibitory activity. Endo-a-(1-->4)-polygalacturonase digestion of AR-2IIb indicated that its "ramified" region (rhamnogalacturonan core possessing neutral oligosaccharide side-chains) contained epitopes for anti-AR-2IIb-IgG, but that 2-keto-3-deoxyoctulosonic acid (KDO)-containing regions and oligogalacturonides obtained from AR-2IIb were not recognized by anti-AR-2IIb-IgG. Although carboxyl-reduction of galacturonic acid in the "ramified" region decreased the inhibitory activity of the "ramified" on its reactivity to anti-AR-2IIb, an acidic tetrasaccharide unit in the rhamnogalacturonan core had negligible inhibitory activity.
通过用从北柴胡根部纯化的一种补体激活果胶（AR-2IIb）免疫兔子获得了抗血清，并且通过在固定有AR-2IIb的琼脂糖凝胶和蛋白G-琼脂糖凝胶上进行亲和层析纯化了IgG类的多克隆抗AR-2IIb抗体。AR-2IIb的高碘酸盐氧化显著降低了其对AR-2IIb与抗AR-2IIb-IgG反应性的抑制活性，但AR-2IIb的链霉蛋白酶消化不影响其抑制活性。来自北柴胡、柴胡和甘草的其他药理活性果胶以及来自北柴胡的补体激活果胶阿拉伯半乳聚糖也对AR-2IIb与抗AR-2IIb-IgG的反应性表现出显著的抑制活性，但这些抑制活性低于AR-2IIb。测试的其他果胶、聚半乳糖醛酸、阿拉伯半乳聚糖、半乳聚糖和阿拉伯聚糖具有可忽略不计的抑制活性。AR-2IIb的内切α-(1→4)-聚半乳糖醛酸酶消化表明其“分支”区域（具有中性寡糖侧链的鼠李糖半乳糖醛酸聚糖核心）含有抗AR-2IIb-IgG的表位，但从AR-2IIb获得的含2-酮-3-脱氧辛酸（KDO）的区域和寡聚半乳糖醛酸不被抗AR-2IIb-IgG识别。尽管“分支”区域中半乳糖醛酸的羧基还原降低了“分支”区域对其与抗AR-2IIb反应性的抑制活性，但鼠李糖半乳糖醛酸聚糖核心中的酸性四糖单元具有可忽略不计的抑制活性。
