Duarte A, Ordoñez N, Castañeda E
Instituto Nacional de Salud, Santafé de Bogotá, Colombia.
Rev Inst Med Trop Sao Paulo. 1994 Mar-Apr;36(2):125-30.
Environmental isolation of Cryptococcus neoformans var. gattii was first made in Australia in 1989 by ELLIS. He established a specific association with the tree species Eucalyptus camaldulensis and E. tereticornis. Based on his findings, ELLIS proposed that the fungus could be exported from Australia to others regions, including Colombia, by means of infected seeds. The purpose of this study was to isolate and identify Cryptococcus sp., associated with Eucalyptus trees; this is the first ecological evaluation of C. neoformans var. gattii in our country. A total of 100 Eucalyptus trees, distributed among 13 zones, located in the center, northeast, east and west of Santafé de Bogotá, were studied. Flowers, fruits, leaves, barks and Eucalyptus debris were collected. The samples were processed by extraction with saline solution containing antibiotics, cultured in selective media and the isolates were identified by morphological and biochemical characterístics. Twenty-seven isolates of 9 Cryptococcus sp. were recovered from 21 Eucalyptus trees, from 5 zones. One C. neoformans var. neoformans serotype A was recovered. The Cryptococcus associated with Eucalyptus is important because this is the first study done in our country.
1989年，埃利斯首次在澳大利亚从环境中分离出新型隐球菌格特变种。他确定了该真菌与赤桉和圆锥花桉这两种树种存在特定关联。基于他的研究结果，埃利斯提出该真菌可能会通过受感染的种子从澳大利亚传播到包括哥伦比亚在内的其他地区。本研究的目的是分离和鉴定与桉树相关的隐球菌属；这是我国对新型隐球菌格特变种的首次生态学评估。研究了位于波哥大圣菲市中心、东北部、东部和西部13个区域的共100棵桉树。采集了花、果实、树叶、树皮和桉树木屑。通过用含抗生素的盐溶液提取对样本进行处理，在选择性培养基中培养，并通过形态学和生化特征对分离株进行鉴定。从5个区域的21棵桉树中分离出了9种隐球菌属的27个分离株。分离出了1株新型隐球菌新型变种血清型A。与桉树相关的隐球菌很重要，因为这是我国开展的第一项此类研究。
