Wanderley D M, Gonzales T T, Pereira M S, Nascimento R D, Moraes-Souza H
Superintendência de Controle de Endemias (SUCEN), São Paulo, SP, Brasil.
Rev Saude Publica. 1993 Dec;27(6):430-5. doi: 10.1590/s0034-89101993000600005.
An inquiry with the objective of bringing the study of the hemotherapy situation in the state of S. Paulo, up-to-date was undertaken in 1990 and compared with that carried out in 1988. In 1990 research was undertaken in 62 counties, 48 of which performed blood transfusions through 104 services, with hemocenter participation in 13.5% of these latter. In 1988 the respective figures were 57, 40, 71 and 8.5%. The number of donors and transfusions and prevalence of serological positivity for Chagas' disease were, respectively: 105,170; 79,544 and 1.24%, while in 1988 the corresponding numbers were: 51,614; 49,211 and 1.52%. Evaluating the serological selection it was found that the following percentages of the services performed serological tests: for Chagas' disease (94.2%), syphilis (94.2%), hepatitis (94.2%), HIV (92.3%) and malaria (20.2%) while in 1988 only 69.0%; 70.4%; 67.6%, 63.4% and 16.9%, respectively, of the services utilized these same tests. In respect of the serological trial specific for Chagas' disease the results were: complement fixation-4.9%; latex-4.0%; indirect immunofluoresce-80%; direct hemaglutination-24.0%, indirect hemaglutination-69.0% and ELISA-59.0% of all services. In the preceding inquiry only 14.3% of the services performed the ELISA test, and complement fixation was utilized in 28.6%. The results show that the services are utilizing ever more sensitive techniques in the serological routine. Further it was observed that in 1988, 77.6% of all the services studied used only one technique for the diagnosis of Chagas' disease while in 1990 92.9% utilized two or more different techniques. It is concluded that the quality of the hemotherapy undertaken in the state of S. Paulo has improved significantly.
1990年开展了一项调查，旨在更新圣保罗州血液疗法情况的研究，并与1988年进行的调查作比较。1990年在62个县进行了研究，其中48个县通过104个服务机构进行输血，血液中心参与了其中13.5%的服务机构。1988年的相应数字分别为57个县、40个县、71个服务机构和8.5%。献血者数量、输血量以及恰加斯病血清学阳性率分别为：105,170人、79,544次和1.24%，而1988年的相应数字为：51,614人、49,211次和1.52%。评估血清学筛选情况发现，各服务机构进行血清学检测的百分比如下：恰加斯病（94.2%）、梅毒（94.2%）、肝炎（94.2%）、艾滋病毒（92.3%）和疟疾（20.2%），而1988年只有69.0%、70.4%、67.6%、63.4%和16.9%的服务机构采用了这些相同检测。关于恰加斯病特异性血清学试验，结果为：补体结合试验——4.9%；乳胶凝集试验——4.0%；间接免疫荧光试验——80%；直接血凝试验——24.0%；间接血凝试验——69.0%；酶联免疫吸附测定——59.0%。在前一次调查中，只有14.3%的服务机构进行酶联免疫吸附测定试验，28.6%的机构采用补体结合试验。结果表明，各服务机构在血清学常规检测中采用的技术越来越灵敏。此外还观察到，1988年，所有接受研究的服务机构中77.6%仅使用一种技术诊断恰加斯病，而1990年92.9%的机构采用两种或更多不同技术。得出的结论是，圣保罗州开展的血液疗法质量有了显著提高。
