Moroni M, Privitera G, Lazzarin A, Celano V, Cavalli G
Quad Sclavo Diagn. 1976 Jun;12(2):207-14.
The immunofluorescente technique detecting antibody-coated bacteria for diagnosis of site of UTI has been applied to 52 urine samples coming from : 11 patients with clinical diagnosis of pylonephritis, 21 infections of the lower urinary tract, 8 asymptomatic bacteriurias and 2 samples of urine obtained directly by pyelocentesis. The technique confirmed to be specific and sensible: strongly fluorescent germs were observed only in samples coming from pyelonephritis or urine from pyelocentesis. Repeatedly, from single samples were isolated more bacterial strains; only one of them was present in significant quantity. The some observation has been made also in 5 patients clinically affected with P.N., where 2 strains were isolated from each sample. In only one sample both strains were fluorescent, white in the remaining 4 samples the strain present in insignificative quantity was fluorescent. These observations, rather than a double localization (pyelo-renal and lower urinary tract) with different aetiologic agents, were interpreted to be the outcome of repeated antibiotic treatments. It is possible that they could have reduced the fluorescent "bacterial load" of the pyelo-renal region under significant number, simultaneously facilitating the emergence of superinfecting germs at the level of the lower urinary tract. We discuss the therapeutic involvements for a proper antibiotic treatment and the usefulness of flanking the test at the quantitative urine culture.
免疫荧光技术检测抗体包被细菌用于诊断尿路感染部位，已应用于52份尿液样本，这些样本来自：11例临床诊断为肾盂肾炎的患者、21例下尿路感染患者、8例无症状菌尿患者以及2份通过肾盂穿刺直接获取的尿液样本。该技术被证实具有特异性和敏感性：仅在来自肾盂肾炎或肾盂穿刺尿液的样本中观察到强荧光细菌。多次从单个样本中分离出更多细菌菌株；其中只有一种数量显著。在5例临床诊断为肾盂肾炎的患者中也有同样的观察结果，从每个样本中分离出2种菌株。仅在一个样本中两种菌株都有荧光，在其余4个样本中数量无显著意义的菌株有荧光。这些观察结果，与其解释为不同病原体导致的双重定位（肾盂 - 肾和下尿路），不如说是反复抗生素治疗的结果。有可能它们降低了肾盂 - 肾区域低于显著数量的荧光“细菌负荷”，同时促进了下尿路部位超级感染病菌的出现。我们讨论了适当抗生素治疗的治疗意义以及在定量尿培养时辅助该检测的有用性。
