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[单克隆抗体在流感病毒检测中的应用]

[The use of monoclonal antibodies for detecting the influenza virus].

作者信息

Bakova A A, Brondz B D, Fedorkina O A, Gusev V A, Gordienko A I, Krivoshein Iu S, Krivorutchenko Iu L, Prokopenko T G, Popov I A

出版信息

Vopr Virusol. 1994 Jul-Aug;39(4):187-9.

PMID:7998401
Abstract

The possibilities of using influenza A (Leningrad) 385/80 (H3N2) virus matrix protein-specific FITC-labeled D8 monoclonal antibodies in immunofluorescence assays were investigated. The virus antigen accumulation was detected in chorioallantoic cells of chick embryos. Exhibiting the type-specific properties, the fluorescent antibodies stain the perinuclear space, cytoplasmic membrane, and granular structures in the cytoplasm of infected cells. The haemagglutination test tires in the corresponding specimens were at least 1:16.

摘要

研究了在免疫荧光试验中使用甲型流感病毒(列宁格勒)385/80(H3N2)病毒基质蛋白特异性异硫氰酸荧光素标记的D8单克隆抗体的可能性。在鸡胚绒毛尿囊细胞中检测到病毒抗原积累。荧光抗体具有型特异性,可对感染细胞细胞质中的核周间隙、细胞质膜和颗粒结构进行染色。相应标本中的血凝试验效价至少为1:16。

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1
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Vopr Virusol. 1994 Jul-Aug;39(4):187-9.
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J Clin Microbiol. 1986 Dec;24(6):907-12. doi: 10.1128/jcm.24.6.907-912.1986.
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Simultaneous determination of the level of antibodies to influenza virus surface and internal proteins by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法同时测定流感病毒表面和内部蛋白的抗体水平。
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Characterization and immunological properties of influenza A virus nucleoprotein (NP): cell-associated NP isolated from infected cells or viral NP expressed by vaccinia recombinant virus do not confer protection.甲型流感病毒核蛋白(NP)的特性及免疫特性:从感染细胞中分离得到的细胞相关NP或由痘苗重组病毒表达的病毒NP均不能提供保护。
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