Rahmah N, Anuar A K, Karim R, Mehdi R, Sinniah B, Omar A W
Dept. of Microbiol. and Parasitol., School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan.
Biochem Biophys Res Commun. 1994 Nov 30;205(1):202-7. doi: 10.1006/bbrc.1994.2650.
Sera from fifty subjects with different presentations of Brugian filariasis and from common soil-transmitted helminth infections were tested for specific anti-filarial IgG and its subclasses. Anti-filarial IgG, IgG1 and IgG3 showed cross-reactivities with soil-transmitted helminthic infections and no significant differences in optical densities among the various groups of filarial patients. In comparison with other groups of subjects, IgG4-ELISA of sera from microfilaraemic patients and some previously microfilaraemic patients showed a significant increase in optical density readings, while IgG2-ELISA showed elevated optical density readings in sera of patients with chronic elephantiasis. Therefore IgG2-ELISA is potentially useful in the diagnosis of brugian chronic elephantiasis while IgG4-ELISA may be beneficial for follow-up diagnosis of treated microfilaraemic patients.
