Hemat F, McEntee K
Laboratory of Structural Biology and Molecular Medicine, UCLA School of Medicine 90024.
Biochem Biophys Res Commun. 1994 Nov 30;205(1):475-81. doi: 10.1006/bbrc.1994.2690.
We have developed a simple PCR based approach for synthesizing high-molecular-weight DNA multimers of short oligonucleotide elements in yields sufficient for preparing DNA affinity columns or for using as probes to detect sequence-specific DNA binding proteins. The technique is rapid, efficient and produces multimers consisting of 30-100 repeating units.
我们已经开发出一种基于聚合酶链式反应（PCR）的简单方法，用于合成短寡核苷酸元件的高分子量DNA多聚体，其产量足以制备DNA亲和柱或用作检测序列特异性DNA结合蛋白的探针。该技术快速、高效，可产生由30至100个重复单元组成的多聚体。
