Garcia M J, Guilhot C, Lathigra R, Menendez M C, Domenech P, Moreno C, Gicquel B, Martin C
Dept Medicina Preventiva, Facultad de Medicina, Universidad Autonoma, Madrid, Spain.
Microbiology (Reading). 1994 Oct;140 ( Pt 10):2821-8. doi: 10.1099/00221287-140-10-2821.
A new insertion sequence (IS) has been isolated from Mycobacterium smegmatis. It is 1361 bp long and possesses characteristics of the IS3 family elements. It harbours 32 bp imperfect inverted repeats at its extremities and a 3 bp direct repeat flanks the element, possibly as the result of a transposition event. This IS, IS1137, contains three major ORFs. Two of them, ORF A and ORF B show homologies both at the amino acid sequence level and at the organization level with the ORFs encoding the transposase of the IS3 family elements. IS1137 has a narrow host range and was found only in M. smegmatis and M. chitae. The fact that IS1137 is not present in the M. tuberculosis complex strains makes this element a new candidate for transposon mutagenesis in mycobacteria.
一种新的插入序列（IS）已从耻垢分枝杆菌中分离出来。它长1361 bp，具有IS3家族元件的特征。其末端有32 bp的不完全反向重复序列，元件两侧有一个3 bp的正向重复序列，这可能是转座事件的结果。这个IS，即IS1137，包含三个主要的开放阅读框（ORF）。其中两个，ORF A和ORF B在氨基酸序列水平和组织水平上都与编码IS3家族元件转座酶的ORF具有同源性。IS1137的宿主范围狭窄，仅在耻垢分枝杆菌和希氏分枝杆菌中发现。IS1137不存在于结核分枝杆菌复合群菌株中的这一事实，使得该元件成为分枝杆菌中转座子诱变的新候选对象。
