结核分枝杆菌IS6110与其他分枝杆菌属细菌DNA之间的同源性证明？
Demonstration of homology between IS6110 of Mycobacterium tuberculosis and DNAs of other Mycobacterium spp.?
作者信息
Kent L, McHugh T D, Billington O, Dale J W, Gillespie S H
机构信息
Department of Medical Microbiology, Royal Free Hospital School of Medicine, London, United Kingdom.
出版信息
J Clin Microbiol. 1995 Sep;33(9):2290-3. doi: 10.1128/jcm.33.9.2290-2293.1995.
Abstract
The insertion sequence IS6110 has an important role in diagnostic PCR and typing of Mycobacterium tuberculosis. We have evaluated a one-tube nested PCR which detects IS6110. Positive results were obtained with DNAs from four of four M. tuberculosis isolates, seven of eight M. fortuitum isolates, seven of seven M. avium-M. intracellulare complex isolates, four of five M. kansasii isolates, four of five M. xenopi isolates, two of four M. malmoense isolates, and one of two M. chelonei isolates. These results were confirmed by hybridization of genomic DNA from bp 505 to 685 of the IS6110 from M. tuberculosis H37Rv. Dot blot hybridization of genomic DNAs from these isolates with the same probe cinfirmed the presence of a homologous sequence in these mycobacterial species. These data suggest that false-positive results may be obtained for clinical samples when some methods based on IS6110 are used [corrected].
摘要
插入序列IS6110在结核分枝杆菌的诊断性聚合酶链反应（PCR）及分型中具有重要作用。我们评估了一种检测IS6110的单管巢式PCR。用来自4株结核分枝杆菌分离株中的4株、8株偶然分枝杆菌分离株中的7株、7株鸟分枝杆菌-胞内分枝杆菌复合群分离株中的7株、5株堪萨斯分枝杆菌分离株中的4株、5株蟾分枝杆菌分离株中的4株、4株马尔默分枝杆菌分离株中的2株以及2株龟分枝杆菌分离株中的1株的DNA获得了阳性结果。通过对来自结核分枝杆菌H37Rv的IS6110的505至685碱基对的基因组DNA进行杂交，证实了这些结果。用相同探针将这些分离株的基因组DNA进行斑点印迹杂交，证实了在这些分枝杆菌种中存在同源序列。这些数据表明，当使用一些基于IS6110的方法时，临床样本可能会出现假阳性结果[已校正]。
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