短小芽孢杆菌R 312对戊二腈衍生物的微生物水解作用

Microbial hydrolysis of glutaronitrile derivatives with Brevibacterium sp. R 312.

作者信息

Kerridge A, Parratt J S, Roberts S M, Theil F, Turner N J, Willetts A J

机构信息

Department of Biological Sciences, University of Exeter, Devon, U.K.

出版信息

Bioorg Med Chem. 1994 Jun;2(6):447-55. doi: 10.1016/0968-0896(94)80014-6.

DOI:10.1016/0968-0896(94)80014-6

PMID:8000867

Abstract

The enantiomerically pure (S)-cyano acids 3 and 4 can be obtained by biotransformation with Brevibacterium sp. R 312 of the corresponding prochiral dinitriles 5 and 6, respectively. The hydrolysis is probably a two step process involving a nitrile hydratase and an amidase. In connection with these investigations a facile method for the synthesis of racemic 4-cyano-3-hydroxybutanoic acid derivatives was developed.

摘要

对映体纯的(S)-氰基酸3和4可分别通过短杆菌属R 312对相应的前手性二腈5和6进行生物转化而获得。水解可能是一个涉及腈水合酶和酰胺酶的两步过程。与这些研究相关，开发了一种合成外消旋4-氰基-3-羟基丁酸衍生物的简便方法。