Teixeira A Z, Iacomini M, McCune B, Gorin P A
Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, Brazil.
Carbohydr Res. 1994 Nov 1;264(1):63-71. doi: 10.1016/0008-6215(94)00180-4.
Extraction of Evernia prunastri with hot aqueous alkali solubilized heteropolysaccharide-containing material which was purified via Fehling precipitation. Further workup of this polysaccharide with Cetavlon gave two fractions having related but different structures. Each structure consisted of a 1-->6-linked alpha-D-Man p main chain partially monosubstituted at O-2 with side chains of alpha-D-Gal p and partially disubstituted at O-2 and O-4 with alpha-D-Gal p and beta-D-Gal p, respectively. The fractions differed in that one contained much more uronic acid than the other, with corresponding predominance of side chains containing alpha-D-Glc pA-(1-->3)-D-Glc p, alpha-D-Gal p-(1-->2)-D-Glc p, and beta-D-Gal f units.
用热碱水溶液提取扁枝衣，可溶解含杂多糖的物质，该物质通过费林沉淀法进行纯化。用西曲溴铵对这种多糖进一步处理得到了两个具有相关但不同结构的级分。每个结构都由一个1→6连接的α-D-甘露糖主链组成，该主链在O-2处部分被α-D-半乳糖侧链单取代，在O-2和O-4处分别被α-D-半乳糖和β-D-半乳糖部分双取代。这两个级分的不同之处在于，其中一个比另一个含有更多的糖醛酸，相应地，含有α-D-葡萄糖醛酸-(1→3)-D-葡萄糖、α-D-半乳糖-(1→2)-D-葡萄糖和β-D-半乳糖醛酸单元的侧链占优势。
