Cope G H, Williams M A
Cell Tissue Res. 1980;209(2):315-27. doi: 10.1007/BF00237634.
A detailed stereological analysis has been made of the organelle content of rabbit acinar cells during the restoration of granule stores following extensive degranulation with isoprenaline (IPR). Rabbits were sacrificed 2, 4, 8, 12 and 16h after IPR administration and the volumes and proportions of intracellular organelles were compared with those of untreated glands. At 2h only 5% of cell volume was occupied by secretion granules, but there was already evidence of nascent granule formation. The volume per cell of secretion granules increased in sigmoid fashion and by 16h amounted to 350 micron3/cell, 36% of cell volume, which are values similar to those of the control replete glands. IPR treatment caused some initial swelling of the cells, and there were transient increases in the volumes of several compartments. However, the volume of mitochondrial and lysosomal compartments had returned to control levels by 4h and that of the nucleus by 8h. The greatest increase was in the volume of the rough endoplasmic reticulum which had increased by nearly 70% by 2h and remained enlarged throughout the period of restitution. However, neither the volume nor the proportion of the smooth membraned compartment varied throughout the period of analysis. The results are analysed in the light of the overall response of the cells to IPR and the interaction of the organelles during the synthetic phase of the secretory cycle. They are presented as a basis for ensuing studies of the granule populations and the membrane composition of the cells during the restoration of granule stores.
对用异丙肾上腺素（IPR）进行广泛脱颗粒后兔腺泡细胞颗粒储存恢复过程中的细胞器含量进行了详细的体视学分析。在给予IPR后2、4、8、12和16小时处死兔子，并将细胞内细胞器的体积和比例与未处理腺体的进行比较。在2小时时，仅5%的细胞体积被分泌颗粒占据，但已有新生颗粒形成的证据。分泌颗粒的每细胞体积呈S形增加，到16小时时达到350立方微米/细胞，占细胞体积的36%，这些值与对照充盈腺体的值相似。IPR处理导致细胞最初出现一些肿胀，几个区室的体积有短暂增加。然而，线粒体和溶酶体区室的体积在4小时时已恢复到对照水平，细胞核的体积在8小时时恢复到对照水平。增加最大的是粗面内质网的体积，到2小时时增加了近70%，并在恢复期间一直保持增大。然而，在整个分析期间，光滑膜区室的体积和比例均未发生变化。根据细胞对IPR的总体反应以及分泌周期合成阶段细胞器之间的相互作用对结果进行了分析。这些结果作为后续研究颗粒储存恢复过程中颗粒群体和细胞的膜组成的基础而呈现。
