孤儿转运体的分子克隆。神经递质转运体家族的一个新成员。

Molecular cloning of an orphan transporter. A new member of the neurotransmitter transporter family.

作者信息

Smith K E, Fried S G, Durkin M M, Gustafson E L, Borden L A, Branchek T A, Weinshank R L

机构信息

Synaptic Pharmaceutical Corporation, Paramus, NJ 07652.

出版信息

FEBS Lett. 1995 Jan 2;357(1):86-92. doi: 10.1016/0014-5793(94)01328-x.

DOI:10.1016/0014-5793(94)01328-x

PMID:8001687

Abstract

A complementary DNA clone predicted to encode a novel transporter was isolated from rat brain and the localization of its mRNA was examined. The cDNA, designated rB21a, predicts a protein with 12 putative transmembrane domains that exhibits significant sequence homology with neurotransmitter transporters. Expression studies have not yet identified the endogenous substrate for this transporter, but the presence of rB21a mRNA within the leptomeninges of the brain suggests the transporter may regulate CSF levels of its substrate. The cloning of rB21a provides the means to determine its physiological functions and the potential to design novel, transporter-based therapeutic agents for neurological and psychiatric disorders.

摘要

从大鼠脑中分离出一个预测编码新型转运蛋白的互补DNA克隆，并检测了其mRNA的定位。该cDNA命名为rB21a，预测编码一种具有12个推定跨膜结构域的蛋白质，与神经递质转运蛋白具有显著的序列同源性。表达研究尚未确定该转运蛋白的内源性底物，但rB21a mRNA在脑软脑膜中的存在表明该转运蛋白可能调节其底物的脑脊液水平。rB21a的克隆为确定其生理功能提供了手段，并为设计基于转运蛋白的新型神经和精神疾病治疗药物提供了潜力。