Díaz M O, Pomykala H M, Bohlander S K, Maltepe E, Malik K, Brownstein B, Olopade O I
Department of Medicine, University of Chicago, Illinois 60637.
Genomics. 1994 Aug;22(3):540-52. doi: 10.1006/geno.1994.1427.
A map of the type-I interferon gene cluster located on the short arm of human chromosome 9 (9p) has been constructed using a contig of YAC clones. This map contains 26 interferon (IFN) genes and pseudogenes, and it accounts for all, except one, of the IFN sequences previously reported by other authors, plus a new IFNW pseudogene. The most distal gene on 9p is IFNB, and the most proximal one is IFNWP19. The direction of transcription for the 20 most distal IFN sequences is toward the telomere and for the 6 most proximal sequences, toward the centromere. Several regions of the cluster show evidence of ancestral duplication events. Some of these events may be explained by unequal crossing over between adjacent tandem genes. The location of several breakpoints within the cluster, from deletions associated with leukemias and gliomas, was also determined.
利用酵母人工染色体（YAC）克隆的重叠群构建了位于人类9号染色体短臂（9p）上的I型干扰素基因簇图谱。该图谱包含26个干扰素（IFN）基因和假基因，涵盖了其他作者先前报道的所有IFN序列（仅一个除外），外加一个新的IFNW假基因。9p上最远端的基因是IFNB，最近端的是IFNWP19。20个最远端IFN序列的转录方向朝向端粒，6个最近端序列的转录方向朝向着丝粒。该基因簇的几个区域显示出祖先重复事件的证据。其中一些事件可能是由相邻串联基因之间的不等交换所解释。还确定了该基因簇内几个与白血病和神经胶质瘤相关的缺失断点的位置。
