Camel Biotechnology Center, Presidential Camels and Camel Racing Affairs centre, Department of the President's Affairs, P O Box 17292, Al Ain, United Arab Emirates.
Camel Biotechnology Center, Presidential Camels and Camel Racing Affairs centre, Department of the President's Affairs, P O Box 17292, Al Ain, United Arab Emirates.
Mol Immunol. 2020 Mar;119:132-143. doi: 10.1016/j.molimm.2020.01.020. Epub 2020 Jan 31.
Investigations into the molecular immune response of dromedary camel, a key livestock species of the arid, have been limited due to the lack of species-specific reagents. Here we describe for the first time, the identification and characterization of type I IFNs of dromedary camel, which are the most important cytokines in the innate host immune response against viruses. We cloned camel IFN-α coding sequences and identified a total of eleven subtypes. The canonical IFN-α subtype designated as IFN-α1 contained a 555-bp Open Reading Frame encoding a protein of 184 amino acids. Recombinant IFN-α1 protein was produced in E. coli and purified from inclusion bodies. Recombinant camel IFN-α1 induced the mRNA expression of interferon-stimulated genes (ISGs) in camel kidney cells. The purified protein also showed potent in-vitro antiviral activity against Camelpox Virus in kidney cells. The identified camel IFN-α protein and the subtypes will facilitate a better understanding of the host immune response to viral infections in camel and the development of potential antiviral biologicals for zoonotic diseases for which camel act as a reservoir.
由于缺乏种特异性试剂，对干旱地区关键家畜物种单峰驼的分子免疫反应的研究一直受到限制。在这里，我们首次描述了单峰驼 I 型干扰素的鉴定和特征，I 型干扰素是先天宿主抗病毒免疫反应中最重要的细胞因子。我们克隆了骆驼 IFN-α 编码序列，并鉴定了总共 11 种亚型。命名为 IFN-α1 的典型 IFN-α 亚型包含一个 555bp 的开放阅读框，编码一个 184 个氨基酸的蛋白质。重组 IFN-α1 蛋白在大肠杆菌中表达，并从包涵体中纯化。重组骆驼 IFN-α1 诱导骆驼肾细胞中干扰素刺激基因 (ISGs)的 mRNA 表达。纯化的蛋白对肾细胞中的羊痘病毒也显示出强大的体外抗病毒活性。鉴定出的骆驼 IFN-α 蛋白和亚型将有助于更好地了解骆驼对病毒感染的宿主免疫反应，以及开发针对作为储存宿主的骆驼的人畜共患病的潜在抗病毒生物制剂。
