一个编码甲基化CpG结合蛋白Mecp2的基因在小鼠X染色体上的遗传和物理图谱定位。

Genetic and physical mapping of a gene encoding a methyl CpG binding protein, Mecp2, to the mouse X chromosome.

作者信息

Quaderi N A, Meehan R R, Tate P H, Cross S H, Bird A P, Chatterjee A, Herman G E, Brown S D

机构信息

Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, United Kingdom.

出版信息

Genomics. 1994 Aug;22(3):648-51. doi: 10.1006/geno.1994.1442.

DOI:10.1006/geno.1994.1442

PMID:8001979

Abstract

The methyl CpG binding proteins (MeCP1 and MeCP2) are a class of proteins that bind to templates containing symmetrically methylated CpGs. Using an interspecific backcross segregating a number of X-linked markers, we have localized the Mecp2 gene in mouse to the X chromosome close to the microsatellite marker DXMit1. Detailed physical mapping utilizing an available YAC contig encompassing the DXMit1 locus has localized the Mecp2 gene to a 40-kb region between the L1cam and the Rsvp loci, indicating the probable position of a homologue on the human X chromosome.

摘要

甲基化CpG结合蛋白（MeCP1和MeCP2）是一类与含有对称甲基化CpG的模板结合的蛋白质。利用分离多个X连锁标记的种间回交，我们已将小鼠中的Mecp2基因定位到靠近微卫星标记DXMit1的X染色体上。利用包含DXMit1位点的现有YAC重叠群进行的详细物理图谱分析已将Mecp2基因定位到L1cam和Rsvp位点之间的一个40 kb区域，这表明了人类X染色体上同源物的可能位置。