Parekh N R, Walker A, Roberts S J, Welch S J
Horticulture Research International, Wellesbourne, Warwick, UK.
J Appl Bacteriol. 1994 Nov;77(5):467-75. doi: 10.1111/j.1365-2672.1994.tb04389.x.
Seven bacterial isolates which degraded the herbicide metamitron (3-methyl-4-amino-6-phenyl-1,2,4-triazin-5-one) were obtained from field-enhanced soil by liquid enrichment culture. All isolates appeared to be identical and a representative, 0246b, was identified as a Rhodococcus sp. by cell wall and fatty acid analyses. This isolate degraded metamitron as the sole source of carbon within 24 h at 25 degrees C and this is the first report of a bacterium capable of growing with metamitron as the sole source of carbon. Metamitron was degraded less rapidly when it was the sole source of both carbon and nitrogen. The rate and extent of degradation was affected by the presence and type of additional sources of carbon and nitrogen in the culture medium. In studies with [14C]-phenyl-labelled metamitron Rhodococcus sp. 0246b partly mineralized the phenyl ring.
通过液体富集培养从田间强化土壤中获得了七株能降解除草剂嗪草酮（3-甲基-4-氨基-6-苯基-1,2,4-三嗪-5-酮）的细菌分离株。所有分离株看起来都相同，通过细胞壁和脂肪酸分析将一株代表性菌株0246b鉴定为红球菌属。该分离株在25℃下24小时内将嗪草酮作为唯一碳源进行降解，这是关于一种能以嗪草酮作为唯一碳源生长的细菌的首次报道。当嗪草酮是碳和氮的唯一来源时，其降解速度较慢。培养基中额外碳源和氮源的存在及类型会影响降解的速率和程度。在用[14C]-苯基标记的嗪草酮进行的研究中，红球菌属0246b使苯环部分矿化。
