Rosi I, Vinella M, Domizio P
Dipartimento di Biologia, Difesa, Biotecnologie Agro-Forestali, Università della Basilicata, Potenza, Italy.
J Appl Bacteriol. 1994 Nov;77(5):519-27. doi: 10.1111/j.1365-2672.1994.tb04396.x.
Three hundred and seventeen strains representing 20 species of yeasts were screened for the presence of beta-glucosidase activity. All of the strains of the species Debaryomyces castellii, Deb. hansenii, Deb. polymorphus, Kloeckera apiculata and Hansenula anomala showed beta-glucosidase activity, but only one of 153 strains of Saccharomyces cerevisiae. The other species behaved differently, depending upon the strain. The strains that hydrolysed arbutin were checked to localize the beta-glucosidase activity. A strain of Deb. hansenii exhibited the highest exocellular activity and some wall-bound and intracellular activity. The beta-glucosidase synthesis from this yeast was enhanced by aerobic conditions of growth, was repressed by high glucose concentration (9%) and occurred during exponential growth. The optimum conditions for enzymatic preparations of Deb. hansenii were between pH 4.0 and 5.0 and 40 degrees C. A high concentration of ethanol and glucose did not reduce the enzymatic activity. The enzymatic preparations of Deb. hansenii released monoterpenols and other alcohols from a grape glycoside extract.
对代表20种酵母的317株菌株进行了β-葡萄糖苷酶活性检测。所有的卡氏德巴利酵母、汉逊德巴利酵母、多形德巴利酵母、柠檬克勒克酵母和异常汉逊酵母菌株均显示出β-葡萄糖苷酶活性，但153株酿酒酵母中只有1株有该活性。其他菌种表现各异，取决于菌株。对水解熊果苷的菌株进行检测以定位β-葡萄糖苷酶活性。一株汉逊德巴利酵母表现出最高的胞外活性以及一些细胞壁结合和细胞内活性。该酵母的β-葡萄糖苷酶合成在有氧生长条件下增强，在高葡萄糖浓度（9%）下受到抑制，且在指数生长期发生。汉逊德巴利酵母酶制剂的最佳条件是pH值在4.0至5.0之间，温度为40℃。高浓度的乙醇和葡萄糖不会降低酶活性。汉逊德巴利酵母的酶制剂从葡萄糖苷提取物中释放出单萜醇和其他醇类。
