Agarbati Alice, Canonico Laura, Pecci Tania, Romanazzi Gianfranco, Ciani Maurizio, Comitini Francesca
Department of Life and Environmental Sciences, Marche Polytechnic University, Via Brecce Bianche, 60131 Ancona, Italy.
Department of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, Via Brecce Bianche, 60131 Ancona, Italy.
Microorganisms. 2022 Jan 18;10(2):200. doi: 10.3390/microorganisms10020200.
() is responsible for grape infection and damage to the winemaking and table grape sectors. Although anti- chemicals are available, they are considered unsustainable for resistance phenomenon and adverse effects on the environment and human health. Research is focused on developing alternative approaches, such as exploiting biological control agents (BCAs). In this context, 19 yeasts of the genera , , , and were tested as antimicrobial agents against development.
A combination of in vitro tests based on dual-culture methods, volatile organic compound production assay, laboratory tests on grape berries (punctured and sprayed with yeasts) and field experiments based on yeast treatments on grapes in vineyards allowed the selection of two potential BCAs.
DiSVA 269 and DiSVA 211 exhibited the best ability to contain the development of , showing the severity, the decay and the McKinney index lower than a commercial biological formulation consisting of a mixture of two different strains, which were used as positive controls.
The results indicated that the selected strains were effective BCA candidates to counteract in the field, applying them in the partial or total replacement of conventional treatments.
（）负责葡萄感染以及对酿酒葡萄和鲜食葡萄产业造成损害。尽管有抗（原文此处缺失具体内容）化学药剂可用，但由于抗性现象以及对环境和人类健康的不利影响，它们被认为是不可持续的。研究重点在于开发替代方法，例如利用生物防治剂（BCAs）。在此背景下，对属于（此处缺失具体属名）、（此处缺失具体属名）、（此处缺失具体属名）、（此处缺失具体属名）和（此处缺失具体属名）的19种酵母作为抗（原文此处缺失具体内容）发育的抗菌剂进行了测试。
基于双培养法的体外测试、挥发性有机化合物产生测定、葡萄浆果上的实验室测试（用酵母穿刺和喷洒）以及基于葡萄园葡萄酵母处理的田间试验相结合，从而筛选出两种潜在的生物防治剂。
DiSVA 269和DiSVA 211表现出抑制（原文此处缺失具体内容）发育的最佳能力，其严重程度、腐烂程度和麦金尼指数均低于由两种不同（此处缺失具体菌株名）菌株混合物组成的商业生物制剂，该商业生物制剂用作阳性对照。
结果表明，所选菌株是在田间对抗（原文此处缺失具体内容）的有效生物防治剂候选物，可用于部分或完全替代传统处理方法。
