• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Cloning and expression of the gene encoding the 7Fe type ferredoxin from a thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii.

作者信息

Aono S, Nakamura S, Aono R, Okura I

机构信息

Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Biochem Biophys Res Commun. 1994 Jun 15;201(2):938-42. doi: 10.1006/bbrc.1994.1792.

DOI:10.1006/bbrc.1994.1792
PMID:8003034
Abstract

The structural gene (fdxA) coding for a 7Fe type ferredoxin of a thermophilic hydrogen oxidizing bacterium Bacillus schlegelii has been cloned and sequenced. The fdxA coding region is 231 nucleotides which codes for a 77 amino acids protein with the molecular weight of 8,744 except for iron-sulfur clusters. The fdxA gene has been expressed in E. coli to obtain the recombinant ferredoxin. The recombinant ferredoxin have shown the identical properties to the native one for absorption spectra, and SDS-PAGE.

摘要

相似文献

1
Cloning and expression of the gene encoding the 7Fe type ferredoxin from a thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii.
Biochem Biophys Res Commun. 1994 Jun 15;201(2):938-42. doi: 10.1006/bbrc.1994.1792.
2
Cloning and expression in Escherichia coli of the gene encoding the [2Fe-2S] ferredoxin from Clostridium pasteurianum.
Biochem Biophys Res Commun. 1993 May 14;192(3):1115-22. doi: 10.1006/bbrc.1993.1532.
3
Molecular cloning of the gene encoding a 2Fe-2S ferredoxin from extremely halophilic archaeon Haloarcula japonica strain TR-1.来自极端嗜盐古菌日本嗜盐嗜盐菌TR-1株的编码2Fe-2S铁氧化还原蛋白基因的分子克隆。
Nucleic Acids Symp Ser. 1997(37):109-10.
4
Overexpression in Escherichia coli of the fdxA gene encoding Rhodobacter capsulatus ferredoxin II.编码荚膜红细菌铁氧化还原蛋白II的fdxA基因在大肠杆菌中的过表达。
Protein Expr Purif. 1995 Apr;6(2):176-84. doi: 10.1006/prep.1995.1022.
5
Purification and characterization of a 7Fe ferredoxin from a thermophilic hydrogen-oxidizing bacterium, Bacillus schlegelii.嗜热氢氧化细菌施氏芽孢杆菌中一种七铁铁氧化还原蛋白的纯化与特性分析
J Biochem. 1992 Dec;112(6):792-5. doi: 10.1093/oxfordjournals.jbchem.a123977.
6
Human ferredoxin: overproduction in Escherichia coli, reconstitution in vitro, and spectroscopic studies of iron-sulfur cluster ligand cysteine-to-serine mutants.人铁氧化还原蛋白：在大肠杆菌中的过量表达、体外重组以及铁硫簇配体半胱氨酸到丝氨酸突变体的光谱研究。
Biochemistry. 1996 Jul 23;35(29):9488-95. doi: 10.1021/bi960467f.
7
Expression in Escherichia coli and characterization of a recombinant 7Fe ferredoxin of Rhodobacter capsulatus.荚膜红细菌重组7Fe铁氧化还原蛋白在大肠杆菌中的表达及特性研究
Biochem J. 1992 Aug 15;286 ( Pt 1)(Pt 1):269-73. doi: 10.1042/bj2860269.
8
Synthesis, cloning and expression of a synthetic gene for high potential iron protein from Chromatium vinosum.来自嗜硫红假单胞菌的高电位铁蛋白合成基因的合成、克隆及表达
Biochem Biophys Res Commun. 1993 Dec 30;197(3):1357-62. doi: 10.1006/bbrc.1993.2626.
9
An Isc-type extremely thermostable [2Fe-2S] ferredoxin from Aquifex aeolicus. Biochemical, spectroscopic, and unfolding studies.来自嗜热栖热菌的一种Isc型超嗜热[2Fe-2S]铁氧化还原蛋白。生物化学、光谱学及去折叠研究。
Biochemistry. 2003 Feb 11;42(5):1354-64. doi: 10.1021/bi027116n.
10
Polysaccharide lyase: molecular cloning of gellan lyase gene and formation of the lyase from a huge precursor protein in Bacillus sp. GL1.多糖裂解酶：结冷胶裂解酶基因的分子克隆以及芽孢杆菌GL1中由巨大前体蛋白形成裂解酶的过程。
Arch Biochem Biophys. 1998 Jun 1;354(1):31-9. doi: 10.1006/abbi.1998.0674.

引用本文的文献

1
ldpA encodes an iron-sulfur protein involved in light-dependent modulation of the circadian period in the cyanobacterium Synechococcus elongatus PCC 7942.LdpA编码一种铁硫蛋白，该蛋白参与了细长聚球藻Synechococcus elongatus PCC 7942生物钟周期的光依赖性调节。
J Bacteriol. 2003 Feb;185(4):1415-22. doi: 10.1128/JB.185.4.1415-1422.2003.