Florin T H, Jabbar I A
Mater Hospital Department of Medicine, University of Queensland, South Brisbane, Australia.
J Gastroenterol Hepatol. 1994 Mar-Apr;9(2):112-7. doi: 10.1111/j.1440-1746.1994.tb01228.x.
This study investigated a possible role for primary bile acid in the control of methanogenesis in the human colon. Production of hydrogen and methane was measured in anaerobic faecal cultures derived from faeces of six 'non-methanogenic' and three methanogenic healthy humans. Using a sensitive technique for gas measurement, methane was detected in all faecal cultures, including those from 'non-methanogenic' humans. Bile acid inhibited methanogenesis in a dose-response fashion in the in vitro 'non-methanogenic' and methanogenic faecal cultures. Inhibition was significant at bile acid concentrations > 0.05%. Methanogenesis correlated with methanogen (methanogenic bacteria) numbers. If this inhibition occurs in vivo, then it would explain much of the epidemiology of non-methanogenesis in humans. From an analysis of net hydrogen production by the faecal cultures, it is inferred that bile acid inhibits other hydrogen-consuming bacteria in addition to methanogens. These in vitro data suggest a major role for bile acid in the accumulation of hydrogen gas in the colon. Possible links between bile acid induced accumulation of gas and irritable bowel syndrome are discussed.
本研究调查了初级胆汁酸在控制人类结肠甲烷生成中的可能作用。在来自六名“非产甲烷型”和三名产甲烷型健康人的粪便厌氧培养物中测量了氢气和甲烷的产生。使用一种灵敏的气体测量技术，在所有粪便培养物中都检测到了甲烷，包括来自“非产甲烷型”人的培养物。胆汁酸在体外“非产甲烷型”和产甲烷型粪便培养物中以剂量反应方式抑制甲烷生成。在胆汁酸浓度>0.05%时抑制作用显著。甲烷生成与产甲烷菌（产甲烷细菌）数量相关。如果这种抑制作用在体内发生，那么它将解释人类非产甲烷现象的许多流行病学情况。通过对粪便培养物净氢气产生的分析推断，胆汁酸除了抑制产甲烷菌外，还抑制其他耗氢细菌。这些体外数据表明胆汁酸在结肠氢气积累中起主要作用。讨论了胆汁酸诱导的气体积累与肠易激综合征之间的可能联系。
