Van Muiswinkel W B, Van Soest P L
Immunology. 1976 Jul;31(1):111-8.
To study the T cell-dependent period of the immune response of mouse spleen cells to sheep erythrocytes the co-operation between T and B cells was abrogated at different times during the in vivo or the in vitro response. The abrogation was performed by killing the T cells with anti-theta serum or anti-H-2 serum. The surviving cells were subsequently cultured in vitro and the number of IgM plaque-forming cells was determined each day. The results indicate that T cells play an important role during the first 3 days of the response in vivo. However, during the in vitro response the presence of the T cells is only required during the first 2 days. The difference between the response in vivo and in vitro is probably due to a synchronous start of the plasma cell development in vitro and a more asynchronous start of this process in vivo.
为了研究小鼠脾细胞对绵羊红细胞免疫反应的T细胞依赖期，在体内或体外反应的不同时间废除T细胞和B细胞之间的协作。通过用抗θ血清或抗H-2血清杀死T细胞来实现废除。随后将存活的细胞进行体外培养，并每天测定IgM斑块形成细胞的数量。结果表明，T细胞在体内反应的前3天发挥重要作用。然而，在体外反应中，仅在头2天需要T细胞的存在。体内和体外反应之间的差异可能是由于体外浆细胞发育同步开始，而体内该过程开始更不同步。
