Gómez M M, Pflug I J, Busta F F
Department of Food Science and Nutrition, University of Minnesota, St. Paul.
J Pharm Sci Technol. 1994 Jan-Feb;48(1):16-23.
Dry heat resistance parameters for Neosartorya fischeri ascospores were obtained at 90 degrees C and 95 degrees C under 30%, 40%, 50%, 60%, and 75% relative humidity (RH) conditions. The dry heat treated spores were exposed to saturated water vapor (for 20-24 h at 4 degrees C) prior to recovery in buffer held at two temperatures (0 degrees C and 80 degrees C). Approximately the same level of recovery was obtained at the two buffer temperatures except at the shortest heating times for the heat treatment carried at 30% and 40% RH, where the number of survivors was significantly higher for spores placed in the buffer held at 80 degrees C. The effect of this high temperature was attributed to heat activation of the ascospores that remained dormant during the dry heat treatment conditions mentioned above. The wet heat resistance of N. fischeri ascospores was also determined at temperatures ranging from 82.5 degrees C to 95 degrees C. The results indicate that as the RH decreased, the heat resistance of the ascospores increased. There were about four orders of magnitude difference in the heat resistance between wet heat (100% RH) and the lowest dry heat treatment condition (30% RH).
在90摄氏度和95摄氏度下，于30%、40%、50%、60%和75%相对湿度（RH）条件下，获得了费氏新萨托菌子囊孢子的干热抗性参数。经过干热处理的孢子在恢复至两种温度（0摄氏度和80摄氏度）的缓冲液中之前，先暴露于饱和水蒸气（在4摄氏度下20 - 24小时）。除了在30%和40%相对湿度下进行热处理的最短加热时间外，在两种缓冲液温度下获得的恢复水平大致相同，在这种情况下，置于80摄氏度缓冲液中的孢子存活数量显著更高。这种高温的影响归因于在上述干热处理条件下保持休眠的子囊孢子的热激活。还在82.5摄氏度至95摄氏度的温度范围内测定了费氏新萨托菌子囊孢子的湿热抗性。结果表明，随着相对湿度降低，子囊孢子的耐热性增加。湿热（100%相对湿度）和最低干热处理条件（30%相对湿度）之间的耐热性存在约四个数量级的差异。
