绵羊外周血淋巴细胞的流式细胞术分析
Flow cytometric analysis of ovine peripheral blood lymphocytes.
作者信息
Smith H E, Jacobs R M, Smith C
机构信息
Department of Pathology, University of Guelph, Ontario.
出版信息
Can J Vet Res. 1994 Apr;58(2):152-5.
Leukocyte suspensions were prepared from the peripheral blood of 12 sheep three times at two month intervals beginning at 12 months of age. Monoclonal antibodies and flow cytometric analysis were used to characterize the cells. There were no significant differences over time therefore the data from the three time intervals were pooled. The mean percentages and ranges (minimum to maximum) of the major lymphocyte subtypes were: B-cells (29.6%, 11-50), gamma delta T-cells (36.6%, 22-68), CD4+ T-cells (14.1%, 8-22) and CD8+ T-cells (12.0%, 4-22). Lymphocyte subtype percentages appeared less variable within than between individuals. Two populations of B-cells were noted; one population had more cytoplasm and light scatter characteristics similar to monocytes while a second population of B-cells was more typical of small lymphocytes. The nature of the large B-cells requires further study.
从12只绵羊的外周血中制备白细胞悬液，从12月龄开始，每隔两个月进行三次。使用单克隆抗体和流式细胞术分析对细胞进行表征。随着时间的推移没有显著差异，因此将三个时间间隔的数据合并。主要淋巴细胞亚群的平均百分比和范围（最小值到最大值）为：B细胞（29.6%，11 - 50）、γδT细胞（36.6%，22 - 68）、CD4 + T细胞（14.1%，8 - 22）和CD8 + T细胞（12.0%，4 - 22）。淋巴细胞亚群百分比在个体内部的变异性似乎小于个体之间。观察到两类B细胞群体；一类群体具有更多细胞质，其光散射特征类似于单核细胞，而另一类B细胞群体更具小淋巴细胞的典型特征。大B细胞的性质需要进一步研究。
Zotero 插件