Jacobs R M, Smith H E, Whetstone C A, Suarez D L, Jefferson B, Valli V E
Department of Pathology, University of Guelph, Ontario, Canada.
Vet Res Commun. 1994;18(6):471-82. doi: 10.1007/BF01839424.
Bovine immunodeficiency-like virus (BIV) was passaged in vivo by intraperitoneal transfusion of ovine whole blood. Prior to transfusion, the recipient sheep were given sodium thioglycolate intraperitoneally to induce mild non-suppurative inflammation. The anti-BIV antibody response, haematology, and peripheral blood lymphocyte subsets (B, gamma delta, CD2+, CD4+ and CD8+) of recipient sheep were assessed for one year following transfusion. Passaging was successful since serum anti-BIV antibody responses were detected in 5 of the 6 recipient sheep; 1 of the 5 remained seropositive throughout the study. Lentivirus was not isolated from the recipient sheep, but provirus was detected by the polymerase chain reaction in DNA from peripheral blood leukocytes in 3 of the 5 sheep that seroconverted. In the BIV-inoculated sheep, neutrophils and eosinophils were significantly increased (p < or = 0.05) at 3 months and between 6 and 8 months postinoculation, respectively. B, CD2+ and CD4+ cells and the CD4+/CD8+ ratios were significantly increased (p < or = 0.05) 2 months postinoculation. Mild, transient haematological changes occurred in BIV-exposed sheep, but illness was not detected in the year.
牛免疫缺陷样病毒（BIV）通过腹腔内输注绵羊全血在体内传代。在输血前，给受体绵羊腹腔内注射巯基乙酸钠以诱导轻度非化脓性炎症。在输血后的一年时间里，对受体绵羊的抗BIV抗体反应、血液学指标以及外周血淋巴细胞亚群（B细胞、γδ细胞、CD2⁺细胞、CD4⁺细胞和CD8⁺细胞）进行了评估。传代成功，因为在6只受体绵羊中有5只检测到血清抗BIV抗体反应；在整个研究过程中，5只中的1只保持血清阳性。未从受体绵羊中分离出慢病毒，但在5只发生血清转化的绵羊中，有3只通过聚合酶链反应在外周血白细胞的DNA中检测到前病毒。在接种BIV的绵羊中，中性粒细胞和嗜酸性粒细胞分别在接种后3个月以及6至8个月时显著增加（p≤0.05）。接种后2个月，B细胞、CD2⁺细胞和CD4⁺细胞以及CD4⁺/CD8⁺比值显著增加（p≤0.05）。接触BIV的绵羊出现了轻度、短暂的血液学变化，但在这一年中未检测到疾病。
