Madsen-Bouterse Sally A, Herndon David R, Grossman Paige C, Rivolta Alejandra A, Fry Lindsay M, Murdoch Brenda M, Piel Lindsay M W
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA.
USDA-ARS Animal Disease Research Unit, Pullman, WA 99164, USA.
Microorganisms. 2024 Dec 21;12(12):2658. doi: 10.3390/microorganisms12122658.
Bighorn sheep (BHS) populations have been reported to experience high levels of morbidity and mortality following infection with . This contrasts with the subclinical presentation in domestic sheep (DS). Understanding this difference requires baseline knowledge of pre- and post-infection immune responses of both species. The present study identifies differences in leukocyte phenotypes between adult BHS and DS before and after intranasal inoculation with 1 × 10. Prior to inoculation, BHS were confirmed to have a higher abundance of leukocyte CD14 and serum concentrations of IL-36RA. In contrast, DS had a higher leukocyte abundance of CD16 in addition to previously observed integrin markers and CD172a, as well as greater serum TNF-α concentrations. Within 15 days of inoculation, BHS displayed signs of mild respiratory disease and DNA was detected on nasal swabs using a quantitative PCR; meanwhile, DS exhibited few to no clinical signs and had levels of DNA below the standard curve threshold. Immunologic markers remained relatively consistent pre- and post-inoculation in DS, while BHS demonstrated changes in the peripheral leukocyte expression of CD172a and CD14. Circulating serum IL-36RA decreased and CXCL10 increased within BHS. These findings highlight significant differences in cellular immunity between BHS and DS, raised and housed under similar conditions, prior to and following inoculation with .
据报道，大角羊（BHS）种群在感染[病原体名称未给出]后发病率和死亡率很高。这与家羊（DS）的亚临床症状形成对比。了解这种差异需要了解这两个物种感染前后免疫反应的基线知识。本研究确定了成年大角羊和家羊在经鼻接种1×10[病原体接种量相关内容未完整给出]前后白细胞表型的差异。接种前，确认大角羊白细胞CD14丰度较高，血清IL - 36RA浓度较高。相比之下，家羊除了先前观察到的整合素标记物和CD172a外，白细胞CD16丰度较高，血清TNF -α浓度也更高。接种后15天内，大角羊出现轻度呼吸道疾病症状，使用定量PCR在鼻拭子上检测到[病原体名称未给出]DNA；与此同时，家羊表现出很少或没有临床症状，[病原体名称未给出]DNA水平低于标准曲线阈值。在家羊中，免疫标记物在接种前后保持相对一致，而大角羊外周白细胞CD172a和CD14的表达发生了变化。大角羊循环血清中IL - 36RA降低，CXCL10升高。这些发现突出了在相似条件下饲养的大角羊和家羊在接种[病原体名称未给出]前后细胞免疫方面的显著差异。
