Kemp M, Hey A S, Kurtzhals J A, Christensen C B, Gaafar A, Mustafa M D, Kordofani A A, Ismail A, Kharazmi A, Theander T G
Centre for Medical Parasitology, University of Copenhagen, Denmark.
Clin Exp Immunol. 1994 Jun;96(3):410-5. doi: 10.1111/j.1365-2249.1994.tb06043.x.
The T cell response to antigens from Leishmania major promastigotes was investigated in peripheral blood mononuclear cells from Sudanese individuals with a history of cutaneous leishmaniasis (CL), Sudanese individuals with positive DTH reaction in the leishmanin skin test but with no history of skin lesions, and in Danes without known exposure to Leishmania parasites. Proliferation and production of interferon-gamma (IFN-gamma) and IL-4 in antigen-stimulated cultures was measured. Lymphocytes from individuals with a history of CL proliferated vigorously and produced IFN-gamma after stimulation with either a crude preparation of L. major antigens or the major surface protease gp63. These cultures produced no or only little IL-4. Also cells from leishmanin skin test-positive donors with no history of CL produced IFN-gamma and no IL-4 in response to L. major antigens. Cells from the unexposed Danes were not activated by gp63. The cells from Danish donors produced either IFN-gamma or IL-4, but not both cytokines after incubation with the crude preparation of L. major antigens. The data show that the T cell response to Leishmania antigens in humans who have had uncomplicated CL or subclinical L. major infection is an IFN-gamma-producing Th1-like response.
对来自硕大利什曼原虫前鞭毛体抗原的T细胞反应，在有皮肤利什曼病（CL）病史的苏丹人、利什曼原虫素皮肤试验呈阳性反应但无皮肤病变病史的苏丹人以及无已知利什曼原虫寄生虫暴露史的丹麦人的外周血单个核细胞中进行了研究。测定了抗原刺激培养物中干扰素-γ（IFN-γ）和IL-4的增殖和产生情况。有CL病史的个体的淋巴细胞在用硕大利什曼原虫抗原粗制品或主要表面蛋白酶gp63刺激后，增殖旺盛并产生IFN-γ。这些培养物不产生或仅产生少量IL-4。同样，来自无CL病史的利什曼原虫素皮肤试验阳性供体的细胞，在对硕大利什曼原虫抗原的反应中产生IFN-γ且不产生IL-4。未暴露的丹麦人的细胞未被gp63激活。丹麦供体的细胞在用硕大利什曼原虫抗原粗制品孵育后，要么产生IFN-γ，要么产生IL-4，但不会同时产生这两种细胞因子。数据表明，在患有单纯性CL或亚临床硕大利什曼原虫感染的人类中，对利什曼原虫抗原的T细胞反应是一种产生IFN-γ的Th1样反应。
