Olobo J O, Anjili C O, Gicheru M M, Mbati P A, Kariuki T M, Githure J I, Koech D K, McMaster W R
Institute of Primate Research, Nairobi, Kenya.
Vet Parasitol. 1995 Dec;60(3-4):199-212. doi: 10.1016/0304-4017(95)00788-6.
Vervet monkeys (Cercopithicus aethiops) were shown to give a positive delayed-type hypersensitivity (DTH) reaction to gp63, a major surface glycoprotein of Leishmania parasites, and also produce antibodies to the molecule following a triple vaccination with a total dose of 150 micrograms of recombinant gp63 mixed with Bacille Calmette Guerin (BCG). However, peripheral blood leucocytes (PBL) from these animals neither proliferated nor produced any interferon-gamma (IFN-gamma) following in vitro stimulation with the antigen. Analysis of lymphocyte subsets following vaccination did not reveal any striking phenotypic alteration of cellular sub-populations in PBL. When vaccinated animals were rechallenged, via the needle, with virulent Leishmania major promastigotes containing salivary gland extracts from vector sandflies, only partial protection was achieved. We concluded from these studies that rgp63 produced in Escherichia coli is a safe vaccine molecule which gives only partial protection following vaccination in the vervet monkey host. The molecule requires further improvement for vaccine and/or immunodiagnosis application.
研究表明，绿猴（Cercopithicus aethiops）对利什曼原虫主要表面糖蛋白gp63呈现阳性迟发型超敏反应（DTH），在用总量为150微克的重组gp63与卡介苗（BCG）混合进行三次接种后，也会产生针对该分子的抗体。然而，这些动物的外周血白细胞（PBL）在体外用该抗原刺激后既不增殖也不产生任何γ干扰素（IFN-γ）。对接种后淋巴细胞亚群的分析未发现PBL中细胞亚群有任何显著的表型改变。当对接种动物经皮下再次用含有来自媒介白蛉唾液腺提取物的强毒利什曼原虫前鞭毛体进行攻击时，仅获得部分保护。我们从这些研究中得出结论，在大肠杆菌中产生的rgp63是一种安全的疫苗分子，在绿猴宿主接种后仅提供部分保护。该分子在疫苗和/或免疫诊断应用方面需要进一步改进。
