Marchiori G E, Briggs R, Arnold J M
Department of Medicine, Victoria Hospital, London, Ontario.
Clin Invest Med. 1994 Apr;17(2):77-87.
Fourteen normal subjects (18-42 y) were studied using venous occlusion plethysmography to investigate the characteristics and reproducibility of the forearm vasoconstrictor response following a prolonged venous occlusion stimulus. Upon release of the stimulus, a transient vasodilation is followed by a vasoconstriction. As the stimulus of venous occlusion pressure increased (40, 60, 80, and 100 mmHg for 5 min), there was a significant increase in maximum flow (p = 0.01). Minimum relative flow (minimum divided by control) was unchanged except at the highest occlusion pressure (0.49 +/- 0.05, 0.38 +/- 0.04, 0.47 +/- 0.07, 0.66 +/- 0.12, p = 0.04). As venous occlusion duration increased (3, 4, 5, and 6 min at 80 mmHg), there was no significant change in minimum relative flow (p = 0.73). With increasing ambient temperature (18, 23, and 28 degrees C), there were no significant changes in maximum or minimum relative flows (p = 0.40 and 0.25, respectively). Minimum relative flow was not significantly altered following occlusion with a 2.5-cm cuff vs. a 10-cm cuff (p = 0.77). Thus, the vasoconstriction response was reproducible over a range of venous occlusion stimuli and experimental conditions. We conclude that dynamic vascular responsiveness to prolonged venous occlusion can be measured by venous plethysmography and that calculation of the minimum flow relative to control flow is an appropriate and reproducible index of the vasoconstrictor response. Based on our studies and to conveniently measure these changes, we recommend a 10-cm venous occlusion cuff, with a pressure < or = 80 mmHg, for durations < or = 5 min, in an ambient temperature of 23 degrees C.
十四名正常受试者（18 - 42岁）采用静脉阻断体积描记法进行研究，以探究长时间静脉阻断刺激后前臂血管收缩反应的特征及可重复性。在解除刺激后，先是出现短暂的血管舒张，随后是血管收缩。随着静脉阻断压力刺激增加（40、60、80和100 mmHg，持续5分钟），最大血流量显著增加（p = 0.01）。最小相对血流量（最小值除以对照值）除在最高阻断压力时外无变化（0.49 ± 0.05、0.38 ± 0.04、0.47 ± 0.07、0.66 ± 0.12，p = 0.04）。随着静脉阻断持续时间增加（80 mmHg时为3、4、5和6分钟），最小相对血流量无显著变化（p = 0.73）。随着环境温度升高（18、23和28摄氏度），最大或最小相对血流量均无显著变化（分别为p = 0.40和0.25）。与使用10厘米袖带阻断相比，使用2.5厘米袖带阻断后最小相对血流量无显著改变（p = 0.77）。因此，在一系列静脉阻断刺激和实验条件下，血管收缩反应具有可重复性。我们得出结论，长时间静脉阻断时动态血管反应性可通过静脉体积描记法测量，相对于对照血流量计算最小血流量是血管收缩反应的一个合适且可重复的指标。基于我们的研究并为方便测量这些变化，我们推荐使用10厘米静脉阻断袖带，压力≤80 mmHg，持续时间≤5分钟，环境温度为23摄氏度。
