Kobayashi F, Sagawa N, Fujii S, Mori T, Endo K
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
Gynecol Obstet Invest. 1994;37(3):199-203. doi: 10.1159/000292559.
CA125 and CA130 in various tissues were characterized using the monoclonal antibodies OC125, 130-22, and 145-9. CA125 and CA130 were eluted in the same fraction in 130-22 affinity chromatography. A sandwich immunoradiometric assay using these antibodies in combination was established. When the solid phase of the assay was changed from OC125 to 145-9, the amount of 125I-OC125 that reacted with CA125 in the amnion increased more than that in other samples. On SDS-PAGE, CA125 and CA130 epitopes in the amnion migrated to different positions. These results indicate that CA125 and CA130 are located on different subunits of the same glycoprotein, and that their synthesis is regulated differently in various tissues.
使用单克隆抗体OC125、130 - 22和145 - 9对各种组织中的CA125和CA130进行了表征。在130 - 22亲和色谱中，CA125和CA130在同一馏分中被洗脱。建立了使用这些抗体组合的夹心免疫放射分析方法。当分析的固相从OC125变为145 - 9时，羊膜中与CA125反应的125I - OC125的量比其他样品增加得更多。在SDS - PAGE上，羊膜中的CA125和CA130表位迁移到不同位置。这些结果表明，CA125和CA130位于同一糖蛋白的不同亚基上，并且它们在各种组织中的合成受到不同的调节。
