Lewis J E, Jensen P J, Wheelock M J
Department of Biology, University of Toledo, Ohio 43606.
J Invest Dermatol. 1994 Jun;102(6):870-7. doi: 10.1111/1523-1747.ep12382690.
Elevation of the calcium concentration in keratinocyte culture induces the rapid organization of adherens junctions and desmosomes. Formation of these intercellular junctions is accompanied by reorganization of the cytoskeleton and, with a more delayed timecourse, stratification of the culture into a multilayered epithelial cell sheet. Keratinocytes express two cadherins, known as E- and P-cadherin, which are the cell-cell adhesion molecules of the adherens junction. Antibody that blocks E-cadherin function delays the calcium-induced formation of both adherens junctions and desmosomes and leads to an abnormally stratified cultured. In the present study, we show that anti-E-cadherin plus anti-P-cadherin antibodies inhibit the formation of adherens junctions and desmosomes, prevent reorganization of the cytoskeleton, and block stratification. These studies indicate that cadherin function is required for the calcium-induced intercellular junction organization and stratification of human keratinocytes in vitro.
角质形成细胞培养物中钙浓度的升高会诱导黏附连接和桥粒的快速形成。这些细胞间连接的形成伴随着细胞骨架的重组，并且在更延迟的时间进程中，培养物分层形成多层上皮细胞片。角质形成细胞表达两种钙黏蛋白，即E-钙黏蛋白和P-钙黏蛋白，它们是黏附连接的细胞间黏附分子。阻断E-钙黏蛋白功能的抗体可延迟钙诱导的黏附连接和桥粒的形成，并导致培养物异常分层。在本研究中，我们表明抗E-钙黏蛋白加抗P-钙黏蛋白抗体可抑制黏附连接和桥粒的形成，阻止细胞骨架的重组，并阻断分层。这些研究表明，钙黏蛋白功能是体外钙诱导的人角质形成细胞间连接组织和分层所必需的。
