Libeau G, Diallo A, Colas F, Guerre L
Vet Rec. 1994 Mar 19;134(12):300-4. doi: 10.1136/vr.134.12.300.
An immunocapture ELISA for the diagnosis of rinderpest and peste des petits ruminants is described. Monoclonal antibodies directed against non-overlapping antigenic domains on the nucleocapsid (N) were used to detect the virus N protein in supernatants from infected cells and in field specimens. The assay, which is very sensitive, can be performed in one hour on pre-coated plates. There was no cross reaction between the two viruses in the test and the N protein could be detected in infected cell supernatants kept at ambient temperature for one week. These results show that the ELISA is suitable for routine diagnosis of field samples.
本文描述了一种用于诊断牛瘟和小反刍兽疫的免疫捕获酶联免疫吸附测定法（ELISA）。针对核衣壳（N）上不重叠抗原结构域的单克隆抗体被用于检测感染细胞上清液和野外样本中的病毒N蛋白。该检测方法非常灵敏，可在预包被板上1小时内完成。在检测中两种病毒之间没有交叉反应，并且在室温下保存一周的感染细胞上清液中仍可检测到N蛋白。这些结果表明该ELISA适用于野外样本的常规诊断。
