哺乳动物中的赖氨酸代谢。
Lysine metabolism in mammals.
作者信息
Fellows F C, Lewis M H
出版信息
Biochem J. 1973 Oct;136(2):329-34. doi: 10.1042/bj1360329.
Abstract
The enzymes involved in the initial degradative steps of lysine metabolism, lysine-2-oxoglutarate reductase and saccharopine dehydrogenase, were studied and their activities in different mammals compared. Values obtained in human, rat, pig, dog, cat, ox and sheep liver indicated that in vitro, appreciable degradation of lysine to saccharopine (4-6nmol/min per mg of protein) occurred. The specific activity of saccharopine dehydrogenase in most species studied was higher than that of lysine-oxoglutarate reductase. The rate of production of glutamate from saccharopine in each animal species was investigated and related to the rate of production of alpha-aminoadipate. The rate of formation of lysine from saccharopine, catalysed by saccharopine oxidoreductase, was examined and correlated with the dietary intake of lysine in each species studied.
摘要
对赖氨酸代谢初始降解步骤中涉及的酶，即赖氨酸-2-氧代戊二酸还原酶和酵母氨酸脱氢酶进行了研究，并比较了它们在不同哺乳动物中的活性。在人、大鼠、猪、狗、猫、牛和羊肝脏中获得的值表明，在体外，赖氨酸可大量降解为酵母氨酸（每毫克蛋白质4-6纳摩尔/分钟）。在所研究的大多数物种中，酵母氨酸脱氢酶的比活性高于赖氨酸-氧代戊二酸还原酶。研究了每种动物物种中从酵母氨酸产生谷氨酸的速率，并将其与α-氨基己二酸的产生速率相关联。检测了由酵母氨酸氧化还原酶催化从酵母氨酸形成赖氨酸的速率，并将其与所研究的每个物种的赖氨酸饮食摄入量相关联。
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本文引用的文献
1
The lysine requirements of the adult rat.成年大鼠对赖氨酸的需求量。
Biochem J. 1945;39(2):200-2. doi: 10.1042/bj0390200.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
epsilon-lysine acylase.ε-赖氨酸酰基转移酶
Arch Biochem Biophys. 1957 Jul;69:56-66. doi: 10.1016/0003-9861(57)90472-1.
4
The amino acid requirements of man. XV. The valine requirement; summary and final observations.人类的氨基酸需求。十五。缬氨酸需求；总结与最终观察结果。
J Biol Chem. 1955 Dec;217(2):987-95.
5
Saccharopine, a product of lysine breakdown by mammalian liver.酵母氨酸，一种哺乳动物肝脏中赖氨酸分解的产物。
Biochem Biophys Res Commun. 1965 Jul 26;20(3):285-90. doi: 10.1016/0006-291x(65)90361-x.
6
Familial hyperlysinemia with lysine-ketoglutarate reductase insufficiency.伴有赖氨酸-酮戊二酸还原酶功能不全的家族性高赖氨酸血症
J Clin Invest. 1969 Aug;48(8):1447-52. doi: 10.1172/JCI106110.
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Saccharopinuria: a new inborn error of lysine metabolism.尿 saccharopine：赖氨酸代谢的一种新的先天性代谢缺陷。
Nature. 1968 May 18;218(5142):679. doi: 10.1038/218679a0.
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The conversion of L-lysine to saccharopine and alpha-aminoadipate in mouse.小鼠体内L-赖氨酸向saccharopine和α-氨基己二酸的转化。
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Saccharopine cleavage by a dehydrogenase of human liver.人肝脏脱氢酶对酵母氨酸的裂解作用。
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Biochim Biophys Acta. 1968 Aug 6;165(1):113-20. doi: 10.1016/0304-4165(68)90195-5.
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