Fellows F C, Lewis M H
Biochem J. 1973 Oct;136(2):329-34. doi: 10.1042/bj1360329.
The enzymes involved in the initial degradative steps of lysine metabolism, lysine-2-oxoglutarate reductase and saccharopine dehydrogenase, were studied and their activities in different mammals compared. Values obtained in human, rat, pig, dog, cat, ox and sheep liver indicated that in vitro, appreciable degradation of lysine to saccharopine (4-6nmol/min per mg of protein) occurred. The specific activity of saccharopine dehydrogenase in most species studied was higher than that of lysine-oxoglutarate reductase. The rate of production of glutamate from saccharopine in each animal species was investigated and related to the rate of production of alpha-aminoadipate. The rate of formation of lysine from saccharopine, catalysed by saccharopine oxidoreductase, was examined and correlated with the dietary intake of lysine in each species studied.
对赖氨酸代谢初始降解步骤中涉及的酶，即赖氨酸-2-氧代戊二酸还原酶和酵母氨酸脱氢酶进行了研究，并比较了它们在不同哺乳动物中的活性。在人、大鼠、猪、狗、猫、牛和羊肝脏中获得的值表明，在体外，赖氨酸可大量降解为酵母氨酸（每毫克蛋白质4-6纳摩尔/分钟）。在所研究的大多数物种中，酵母氨酸脱氢酶的比活性高于赖氨酸-氧代戊二酸还原酶。研究了每种动物物种中从酵母氨酸产生谷氨酸的速率，并将其与α-氨基己二酸的产生速率相关联。检测了由酵母氨酸氧化还原酶催化从酵母氨酸形成赖氨酸的速率，并将其与所研究的每个物种的赖氨酸饮食摄入量相关联。
