Rochholz G, Ahrens B, Schütz H
Institute of Legal Medicine, University of Giessen, Fed. Rep. of Germany.
Arzneimittelforschung. 1994 Apr;44(4):469-71.
A modified thin-layer chromatographic method with fluorescence detection is described for the screening of flunitrazepam and its major metabolites in urine. The method is based on the hydrolysis and subsequent formation of acridine derivatives by cyclization in dimethyl-formamide. This reliable screening procedure, which has proven its usefulness in many laboratories, provides valid results in the absence of diclofenac, carbamazepine, and tricyclic antidepressants. On the other hand these latter substances are frequently detectable in urine samples taken from addicts especially during therapy. So it was necessary to modify the procedure.
本文描述了一种用于筛查尿液中氟硝西泮及其主要代谢物的改良薄层色谱荧光检测法。该方法基于在二甲基甲酰胺中环化水解并随后形成吖啶衍生物。这种可靠的筛查程序已在许多实验室证明了其有效性，在不存在双氯芬酸、卡马西平和三环类抗抑郁药的情况下可提供有效结果。另一方面，在尤其是治疗期间从成瘾者采集的尿液样本中，这些物质经常可检测到。因此有必要对该程序进行修改。
