Markel P D, Johnson T E
Department of Psychology, CB 447 University of Colorado, Boulder 80309-0447.
Mamm Genome. 1994 Apr;5(4):199-202. doi: 10.1007/BF00360545.
We are mapping quantitative trait loci (QTLs) that influence ethanol-induced anesthesia (sleep time) in the Long-sleep (LS) and Short-sleep (SS) selected lines of mice. Fifty microsatellite-STS markers were initially screened for simple-sequence length polymorphisms between the LS and SS lines. Nineteen markers were polymorphic. Eleven markers unequivocally differentiated the LS and SS lines and were used to establish strain distribution patterns for the LSXSS series of recombinant inbred strains. Five markers each accounted for at least 5% of sleep-time genetic variance among the RI strains. Linkage of provisional QTLs detected among RIs will be confirmed or disproved in a large F2 population. This ongoing QTL-mapping project eventually will result in a strain distribution pattern for the LSXSS RI series with an average marker spacing of 5 centimorgans.
我们正在绘制影响长睡眠（LS）和短睡眠（SS）品系小鼠乙醇诱导麻醉（睡眠时间）的数量性状基因座（QTL）。最初对50个微卫星-STS标记进行筛选，以寻找LS和SS品系之间的简单序列长度多态性。19个标记具有多态性。11个标记明确区分了LS和SS品系，并用于建立重组近交系LSXSS系列的品系分布模式。在重组近交系中，5个标记各自至少占睡眠时间遗传方差的5%。在重组近交系中检测到的临时QTL的连锁关系将在一个大的F2群体中得到证实或否定。这个正在进行的QTL定位项目最终将产生LSXSS重组近交系系列的品系分布模式，平均标记间距为5厘摩。
