Markel P D, Fulker D W, Bennett B, Corley R P, DeFries J C, Erwin V G, Johnson T E
Institute for Behavioral Genetics, University of Colorado, Boulder 80309-0447, USA.
Behav Genet. 1996 Jul;26(4):447-58. doi: 10.1007/BF02359489.
We are mapping the genes (quantitative trait loci or QTLs) that are responsible for individual differences in ethanol sensitivity, measured as the duration of loss of righting, reflex (LORR) and blood ethanol concentrations upon recovery of the righting reflex (BEC). The Long-Sleep (LS) and Short-Sleep (SS) selected lines of mice manifest an 18-fold difference in LORR and serve as a rodent model for ethanol sensitivity. The LS x SS recombinant inbred (RI) series, developed from LS and SS lines, are an important resource for QTL mapping of ethanol-related responses. The current report summarizes the initial QTL analysis of LORR and BEC in the LS x SS strains and compares the results of correlational analysis with an interval-mapping approach. The data provide strong evidence for QTLs that influence ethanol sensitivity on mouse chromosomes 1 and 2 and possible QTLs on chromosomes 1, 3, 4, 5, 6, 7, 12, 13, 16, and 18. These results are compared to those from an F2 cross which confirms QTLs on chromosomes 1, 2, 4, and 18.
我们正在绘制那些导致乙醇敏感性个体差异的基因（数量性状基因座或QTL），乙醇敏感性通过翻正反射丧失持续时间（LORR）以及翻正反射恢复时的血液乙醇浓度（BEC）来衡量。长睡眠（LS）和短睡眠（SS）小鼠选择品系在LORR上表现出18倍的差异，并作为乙醇敏感性的啮齿动物模型。由LS和SS品系培育出的LS×SS重组近交（RI）系列，是用于乙醇相关反应QTL定位的重要资源。本报告总结了LS×SS品系中LORR和BEC的初步QTL分析，并将相关分析结果与区间定位方法进行了比较。数据为影响小鼠第1和第2号染色体上乙醇敏感性的QTL以及第1、3、4、5、6、7、12、13、16和18号染色体上可能的QTL提供了有力证据。将这些结果与F2杂交的结果进行了比较，后者证实了第1、2、4和18号染色体上的QTL。
