Sinanjan E S, Kugaevskaja E V
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow.
Biochem Mol Biol Int. 1994 Jan;32(1):173-80.
Recently we have revealed angiotensin-converting enzyme (ACE)-activating factor(s) in human neutrophils and blood. In this study the distribution of ACE-activating factor(s) was examined in various tissues. The effect of supernatants obtained from heated bovine tissue extracts on the activity of purified kidney ACE was studied. Pretreatment of ACE with supernatants or their PM-30 (Amicon) ultrafiltrates induced an increase in the enzyme activity. The maximal ACE activation (70%) was observed in the presence of lung and heart ultrafiltrates; lower activation (about 30-50%) was with kidney and some brain areas ultrafiltrates. In other tissues studied the activating capacity was negligible if any. ACE activity determined in tissue extracts did not correlate with the activating capacity. The results obtained provide evidence for the presence of ACE activator(s) in some bovine tissues. The pathophysiological relevance of the activator is discussed.
