van Furth R, van Zwet T L, Buisman A M, van Dissel J T
Department of Infectious Diseases, University Hospital, Leiden, Netherlands.
J Infect Dis. 1994 Jul;170(1):234-7. doi: 10.1093/infdis/170.1.234.
This study concerns the effect of anti-tumor necrosis factor (TNF) antibodies on the course of a sterile inflammatory reaction in the peritoneum and a generalized infection with gram-positive bacteria. Mice received an intravenous injection of rabbit anti-TNF serum or normal rabbit serum 24 h before an intraperitoneal injection of heat-killed Listeria monocytogenes or an intramuscular injection of live L. monocytogenes. The course of the leukocytes in blood and the peritoneal cavity was followed for 72 h; the infection was evaluated for 144 h. The results lead to the conclusion that anti-TNF inhibits the migration of granulocytes and monocytes from bone marrow to the circulation and from the circulation to the peritoneal cavity during an acute inflammation. Furthermore, treatment of mice with anti-TNF serum enhanced the growth of Listeria monocytogenes in thigh muscle, liver, and spleen. The results of this study indicate that treatment with anti-TNF antibodies can inhibit the development of a cellular inflammatory exudate and can have a deleterious effect on the course of an infection with gram-positive bacteria.
本研究关注抗肿瘤坏死因子（TNF）抗体对腹膜无菌性炎症反应进程以及革兰氏阳性菌全身性感染的影响。在腹腔注射热灭活的单核细胞增生李斯特菌或肌肉注射活的单核细胞增生李斯特菌前24小时，小鼠接受静脉注射兔抗TNF血清或正常兔血清。对血液和腹腔中的白细胞进程进行72小时的跟踪；对感染情况进行144小时的评估。结果得出结论，在急性炎症期间，抗TNF可抑制粒细胞和单核细胞从骨髓向循环系统以及从循环系统向腹腔的迁移。此外，用抗TNF血清治疗小鼠会增强单核细胞增生李斯特菌在大腿肌肉、肝脏和脾脏中的生长。本研究结果表明，用抗TNF抗体治疗可抑制细胞炎性渗出物的形成，并且对革兰氏阳性菌感染进程具有有害影响。
