神经精神性狼疮中的淋巴细胞和神经元抗原：可洗脱、可免疫沉淀的淋巴细胞/神经元52kd反应性的存在。
Lymphocyte and neuronal antigens in neuropsychiatric lupus: presence of an elutable, immunoprecipitable lymphocyte/neuronal 52 kd reactivity.
作者信息
Denburg J A, Behmann S A
机构信息
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
出版信息
Ann Rheum Dis. 1994 May;53(5):304-8. doi: 10.1136/ard.53.5.304.
OBJECTIVE
To examine specific lymphocyte or neuronal antigens immuno-precipitated by systemic lupus erythematosus (SLE) sera.
METHOD
SLE sera were screened for the presence of antibodies binding to surface antigens of CD4(+) HUT-78 or SK-N-SH and IMR-6 neuroblastoma cells using Western blotting or radioimmunoprecipitation.
RESULTS
IgG eluates from both lymphocytes and neuroblastoma cells recognised a 52 kd band in HUT 78 cell lysates. Eight sera studied further using radioimmunoprecipitation also demonstrated binding to a 52 kd antigen (4/8 on HUT-78, 8/8 on SK-N-SH cells), partially depleted by absorption with viable HUT-78.
CONCLUSION
A 52 kd antigen recognised by SLE sera on lymphocytes and neuronal cells may play a role in the pathogenesis of neuropsychiatric-SLE.
目的
检测系统性红斑狼疮（SLE）血清免疫沉淀的特定淋巴细胞或神经元抗原。
方法
采用蛋白质印迹法或放射免疫沉淀法，筛选SLE血清中与CD4(+) HUT-78或SK-N-SH及IMR-6神经母细胞瘤细胞表面抗原结合的抗体。
结果
淋巴细胞和神经母细胞瘤细胞的IgG洗脱物在HUT 78细胞裂解物中识别出一条52 kd的条带。进一步用放射免疫沉淀法研究的8份血清也显示与一种52 kd抗原结合（HUT-78细胞上4/8，SK-N-SH细胞上8/8），用活的HUT-78吸收后部分耗尽。
结论
SLE血清在淋巴细胞和神经元细胞上识别的一种52 kd抗原可能在神经精神性SLE的发病机制中起作用。
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