Chen S, Gavish B, Barshtein G, Mahler Y, Yedgar S
Department of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Biochim Biophys Acta. 1994 Jun 22;1192(2):247-52. doi: 10.1016/0005-2736(94)90125-2.
The effect of hydrostatic pressure of up to 15 bars on the aggregability of rat and human red blood cells (RBC), i.e., their capability to form aggregates, was studied using computerized image analysis. The aggregate size distribution was determined under ambient pressure, following application of hydrostatic pressure for various durations up to 2 h. It was found that RBC aggregability markedly increases, up to three-fold, as the pressure which had been applied was increased. Accordingly, higher shear stress is required for dispersing the aggregates of pressure-treated RBC than those of untreated cells. The median size of human RBC aggregates was about three times higher than that of rat RBC, and this ratio was maintained following pressure treatment. RBC aggregability is a major determinant in blood flow, especially in the microcirculation. Pressure at the levels used in this study occurs in physiological states such as hyperbaric treatment or diving. The enhanced aggregability induced by application of such pressure implies that blood flow in microvessels might be altered under conditions associated with elevated hydrostatic pressure.
利用计算机图像分析技术，研究了高达15巴的静水压力对大鼠和人类红细胞（RBC）聚集性的影响，即它们形成聚集体的能力。在环境压力下，以及在施加长达2小时的不同持续时间的静水压力后，测定聚集体大小分布。结果发现，随着施加压力的增加，红细胞聚集性显著增加，最高可达三倍。因此，与未处理细胞相比，分散经压力处理的红细胞聚集体需要更高的剪切应力。人类红细胞聚集体的中位大小约为大鼠红细胞的三倍，且该比例在压力处理后保持不变。红细胞聚集性是血流的主要决定因素，尤其是在微循环中。本研究中使用的压力水平会出现在诸如高压治疗或潜水等生理状态下。施加此类压力所诱导的聚集性增强意味着，在与静水压力升高相关的条件下，微血管中的血流可能会发生改变。
