Wang X, Sun B, Yasuyama K, Salvaterra P M
Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, CA 91010.
Insect Biochem Mol Biol. 1994 Mar;24(3):233-42. doi: 10.1016/0965-1748(94)90002-7.
Antibodies recognizing horse radish peroxidase (HRP) stain neurons in Drosophila and other insects. We have used Western blots to analyze and characterize some of the anti-HRP reactive components from Drosophila melanogaster. Anti-HRP reactive components can be reproducibly detected during all developmental stages, although the pattern changes at different developmental times. In adults, there are at least 10 reproducibly stained components. Two of the bands, with molecular sizes of 42 and 80 kDa are likely to be the major contributors to neuronal anti-HRP staining in Drosophila. These components are enriched in adult fly heads. In contrast, many of the other anti-HRP reactive components in adults are enriched in abdomen and are present exclusively or at much higher levels in male flies. We have purified and characterized two of the male specific components with molecular sizes of 62 and 48 kDa. Partial N-terminal amino acid sequencing revealed that the 62 kDa protein is identical to a part of D. melanogaster carboxylesterase to EC 3.1.1.1) while he 48 kDa protein does not match any known sequences. Esterase-6 has previously been shown to be enriched in male accessory gland and consistent with this we show staining of this structure with anti-HRP antibodies.
识别辣根过氧化物酶（HRP）的抗体可对果蝇和其他昆虫的神经元进行染色。我们已使用蛋白质免疫印迹法来分析和鉴定黑腹果蝇中一些抗HRP反应性成分。在果蝇的所有发育阶段均可重复检测到抗HRP反应性成分，尽管其模式在不同发育时期会发生变化。在成虫中，至少有10种可重复染色的成分。其中两条带，分子量分别为42 kDa和80 kDa，可能是果蝇神经元抗HRP染色的主要成分。这些成分在成年果蝇头部中含量丰富。相比之下，成虫中许多其他抗HRP反应性成分在腹部含量丰富，且仅在雄蝇中存在或含量更高。我们已纯化并鉴定了两种分子量分别为62 kDa和48 kDa的雄性特异性成分。部分N端氨基酸测序显示，62 kDa的蛋白质与黑腹果蝇羧酸酯酶（EC 3.1.1.1）的一部分相同，而48 kDa的蛋白质与任何已知序列均不匹配。酯酶-6先前已被证明在雄性附腺中含量丰富，与此一致的是，我们用抗HRP抗体显示了该结构的染色。
