Jan L Y, Jan Y N
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2700-4. doi: 10.1073/pnas.79.8.2700.
Antibodies specific for horseradish peroxidase (HRPeroxase) bind to neuronal membranes in Drosophila and serve as a specific neuronal marker. Immunocytochemical staining with these antibodies marks sensory neurons, peripheral nerves, and fiber tracks in the central nervous system of embryos, larvae, and adult flies. Similar patterns of staining also were seen in embryos of the grasshopper. It appears that an antigen associated with the nervous system and appearing early in differentiation is recognized by antibodies to HRPeroxase. Using this staining method, we followed embryogenesis of the central nervous system in Drosophila and found that the organization of central fiber tracks resembled that in the previously well-characterized grasshopper. We have used the anti-HRPeroxase antibodies to show that mutations affecting segmentation in Drosophila affect the organization of the embryonic nervous system.
辣根过氧化物酶（HR过氧化物酶）特异性抗体与果蝇的神经元膜结合，并作为一种特异性神经元标记物。用这些抗体进行免疫细胞化学染色可标记胚胎、幼虫和成年果蝇中枢神经系统中的感觉神经元、外周神经和纤维束。在蝗虫胚胎中也观察到了类似的染色模式。似乎与神经系统相关且在分化早期出现的一种抗原可被HR过氧化物酶抗体识别。利用这种染色方法，我们追踪了果蝇中枢神经系统的胚胎发育过程，发现中枢纤维束的组织方式与之前已充分研究的蝗虫相似。我们利用抗HR过氧化物酶抗体表明，影响果蝇体节形成的突变会影响胚胎神经系统的组织。
