Woodhead A P, Khan M A, Stay B, Tobe S S
Department of Biological Sciences, University of Iowa, Iowa City 52242.
Insect Biochem Mol Biol. 1994 Mar;24(3):257-63. doi: 10.1016/0965-1748(94)90005-1.
Allatostatins VI and VII have been isolated from saline extracts of the brain of the viviparous cockroach Diploptera punctata. Active fractions, obtained by successive reverse-phase high pressure liquid chromatography separations, inhibited juvenile hormone (JH) III production by corpora allata (CA) in vitro. The primary structures, Y-P-Q-E-H-R-F-S-F-G-L-amide (VI) and D-G-R-M-Y-S-F-G-L-amide (VII), clearly identify them as members of a family of D. punctata allatostatins, five of which have been identified previously and shown to have F-G-L-amide at the C terminus. Synthetic allatostatins VI and VII coeluted with the native allatostatins on two successive high pressure liquid chromatography separations. Allatostatins VI and VII have the same capacity to inhibit JH production by CA of 2 day virgin females as does allatostatin I, previously shown to be a potent inhibitor similar in activity to allatostatin V. Allatostatins VI and VII also resemble the other allatostatins in their ability to affect both larval and adult CA, in the reversibility of their effect, and in the abolition of their effect by addition of farnesoic acid (a late precursor of JH) to CA in vitro.
咽侧体抑制素VI和VII已从胎生蟑螂双斑大蠊的脑盐提取物中分离出来。通过连续的反相高压液相色谱分离获得的活性组分在体外抑制咽侧体（CA）产生保幼激素（JH）III。其一级结构，Y-P-Q-E-H-R-F-S-F-G-L-酰胺（VI）和D-G-R-M-Y-S-F-G-L-酰胺（VII），明确表明它们是双斑大蠊咽侧体抑制素家族的成员，其中五个先前已被鉴定，并显示在C末端具有F-G-L-酰胺。合成的咽侧体抑制素VI和VII在两次连续的高压液相色谱分离中与天然咽侧体抑制素共洗脱。咽侧体抑制素VI和VII抑制2日龄未交配雌虫的咽侧体产生保幼激素的能力与咽侧体抑制素I相同，咽侧体抑制素I先前已被证明是一种强效抑制剂，其活性与咽侧体抑制素V相似。咽侧体抑制素VI和VII在影响幼虫和成虫咽侧体的能力、其作用的可逆性以及通过在体外向咽侧体中添加法尼酸（保幼激素的晚期前体）来消除其作用方面也与其他咽侧体抑制素相似。
