Greferath U, Grünert U, Müller F, Wässle H
Max-Planck-Institut für Hirnforschung, Frankfurt, Germany.
Cell Tissue Res. 1994 May;276(2):295-307. doi: 10.1007/BF00306115.
The distribution of gamma-aminobutyric acidA (GABAA) receptors in the rabbit retina is investigated and compared with the distribution of GABAergic neurons using immunocytochemical methods. Antibodies against the alpha 1, beta 2/3, and gamma 2 subunits of the GABAA receptor label subpopulations of bipolar, amacrine and ganglion cells. Double labeling experiments show that the gamma 2 subunit is colocalized with the alpha 1 and the beta 2/3 subunits in bipolar, amacrine and ganglion cells. Electron microscopy reveals that in the outer plexiform layer, GABAA receptor immunoreactivity is present on dendrites of cone bipolar cells adjacent to the cone pedicles. Bipolar cell dendrites are also receptor-positive at synapses from interplexiform cells. Some receptor immunoreactivity is found intracellularly in processes of horizontal cells. In the inner plexiform layer, GABAA receptor immunoreactivity is present on both rod bipolar and cone bipolar axon terminals at putative GABAergic input sites. Amacrine and ganglion cell processes in sublamina a and b are also labeled.
采用免疫细胞化学方法研究了兔视网膜中γ-氨基丁酸A（GABAA）受体的分布，并与GABA能神经元的分布进行了比较。针对GABAA受体α1、β2/3和γ2亚基的抗体标记了双极细胞、无长突细胞和神经节细胞的亚群。双重标记实验表明，γ2亚基与α1和β2/3亚基在双极细胞、无长突细胞和神经节细胞中共定位。电子显微镜显示，在外网状层，GABAA受体免疫反应性存在于与视锥细胞蒂相邻的视锥双极细胞的树突上。双极细胞树突在来自网间细胞突触处也呈受体阳性。在水平细胞的突起中发现一些细胞内受体免疫反应性。在内网状层，在假定的GABA能输入部位，GABAA受体免疫反应性存在于视杆双极细胞和视锥双极细胞的轴突终末上。a层和b层中的无长突细胞和神经节细胞突起也被标记。
