Bartley T D, Bogenberger J, Hunt P, Li Y S, Lu H S, Martin F, Chang M S, Samal B, Nichol J L, Swift S
Amgen, Incorporated, Amgen Center, Thousand Oaks, California 91320.
Cell. 1994 Jul 1;77(7):1117-24. doi: 10.1016/0092-8674(94)90450-2.
A novel megakaryocyte growth and development factor (MGDF) has been identified in aplastic canine plasma, and its cDNAs have been cloned from canine, murine, and human sources. Purified canine MGDF isolated by procedures involving MpI receptor affinity chromatography exists in at least two forms, with apparent molecular masses of 25 kDa and 31 kDa, that share the N-terminal amino acid sequence APP-ACDPRLLNKMLRDSHVLH. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. Canine MGDF and recombinant human MGDF support the development of megakaryocytes from human CD34+ progenitor cell populations in liquid culture and promote the survival of a factor-dependent murine cell line (32D) engineered to express MpI. These biological activities are blocked by the soluble extracellular domain of MpI. These data demonstrate that MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPI receptor.
在再生障碍性贫血犬的血浆中已鉴定出一种新型巨核细胞生长和发育因子（MGDF），并且已从犬、小鼠和人类来源克隆出其cDNA。通过涉及MpI受体亲和层析的程序分离得到的纯化犬MGDF至少以两种形式存在，表观分子量分别为25 kDa和31 kDa，它们共享N端氨基酸序列APP - ACDPRLLNKMLRDSHVLH。MGDF的人、犬和小鼠cDNA高度保守，分别编码353、352和356个氨基酸的蛋白质的开放阅读框，包括预测的信号肽。犬MGDF和重组人MGDF在液体培养中支持人CD34 +祖细胞群体中巨核细胞的发育，并促进经基因工程改造以表达MpI的因子依赖性小鼠细胞系（32D）的存活。这些生物学活性被MpI的可溶性细胞外结构域阻断。这些数据表明MGDF是一种调节巨核细胞发育的新型细胞因子，并且是MPI受体的配体。
