Goyanes V, Fernández J L, Pereira S, Campos A, Rodriguez E, González M, Rodriguez M, Espido O, García M
Sección de Genética, Centro Materno-Infantil Teresa Herrera, La Coruña, Spain.
Cytobios. 1994;77(309):67-72.
In order to identify transplacental induced mutagenicity in humans the baseline frequency of sister chromatid exchanges (SCEs) in amniotic fluid cells (8.56 +/- 0.28 per cell) from 14 to 16-week-old foetuses as well as in peripheral blood lymphocytes (7.96 +/- 0.26 per cell) from newborns was determined. A group of twelve patients who were analysed both at 14-16 weeks gestation and at birth showed no SCE variations. These results suggest that foetal amniocytes and T lymphocytes from newborns present a similar sensitivity to the mutagenic activity of the thymidine analogue 5-bromodeoxyuridine employed in the sister chromatid differentiation.
为了确定人类经胎盘诱导的致突变性，测定了14至16周龄胎儿羊水细胞（每细胞8.56±0.28）以及新生儿外周血淋巴细胞（每细胞7.96±0.26）中姐妹染色单体交换（SCE）的基线频率。一组在妊娠14至16周和出生时均接受分析的12名患者未显示SCE变化。这些结果表明，胎儿羊膜细胞和新生儿的T淋巴细胞对用于姐妹染色单体分化的胸腺嘧啶类似物5-溴脱氧尿苷的诱变活性具有相似的敏感性。
